豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义

doi:10.13418/j.issn.1001-165x.2014.01.011

中国临床解剖学杂志 ›› 2014, Vol. 32 ›› Issue (1): 45-47.doi: 10.13418/j.issn.1001-165x.2014.01.011

豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义

王江平^1,2，　王勤章¹，　丁国富¹

1. 石河子大学医学院第一附属医院泌尿外科, 新疆 832000； 2.华中科技大学同济医学院
附属同济医院泌尿外科，武汉 430030

收稿日期:2013-05-01 出版日期:2014-01-25 发布日期:2014-02-11
通讯作者: 王勤章，主任医师，副教授，Tel：0993-2858894，E-mail: wqzl969@sina.com.cn E-mail:mingzhujp@126.com
作者简介:王江平（1980-），男，主治医师，博士，研究方向：泌尿外科学、男科学， Tel：0993-2858894
基金资助:
国家自然科学基金资助项目(81060064)

Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder

WANG Jiang-ping ^1,2, WANG Qin-zhang¹, DING Guo-fu¹

1. Department of Urology, the First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832000，Xinjiang, China；2. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2013-05-01 Online:2014-01-25 Published:2014-02-11

摘要/Abstract

摘要：

目的　观察二聚体Cajal间质细胞(interstitial cells of Cajal，ICC)在豚鼠膀胱不同组织及部位的分布特点，并探讨其意义。方法　电子显微镜下观察豚鼠膀胱壁组织切片黏膜层，黏膜下层，肌层内二聚体ICC分布情况；对膀胱组织切片进行免疫荧光染色，用c-Kit抗体标记ICC，激光共聚焦显微镜下观察二聚体ICC在膀胱顶部、体部、颈部的分布特点。结果　在电子显微镜下见二聚体ICC主要分布于黏膜下层，而肌层主要以单体ICC为主。免疫荧光染色发现每高倍镜视野下膀胱顶部二聚体ICC平均数量为（3.47±0.53）个，体部和颈部为（1.57±0.45）个和(0.49±0.19)个。膀胱顶部二聚体ICC数量明显高于体部和颈部（P<0.01）。结论　二聚体ICC主要分布于豚鼠膀胱顶部的黏膜下层，可能是感受黏膜张力刺激，引发顶部膀胱自发兴奋的起搏细胞。

关键词: 膀胱, Cajal间质细胞, 起搏细胞

Abstract:

Objective　To observe the distribution of dimer interstitial cells of Cajal (ICC) in the different organizations and parts in guinea pig bladder.　Methods　The guinea pig bladder sections were made and the distribution of dimer ICC within different organizations were observed using electron microscope; The cryostat tissue sections of guinea pig bladder were made and dimer ICC marked with c-Kit antibody in different bladder parts were observed with immunofluorescence.　Results　Dimmer ICC, under electron microscope, was mainly distributed in the submucosa of detrusor while monomer ICC was mainly distributed between detrusor bundles. Under high-powered fields of confocal microscopy, the number of dimer ICC in bladder tip was 3.47±0.53 , bladder body was 1.57±0.45 and bladder neck was 0.49±0.19. Dimer ICC density in the bladder tip was significantly higher than that in the bladder body and neck (P <0.01).　Conclusion　Dimer ICC, mainly located in the submucosa of bladder tip, might accept the tension stimulation from mucous and then trigger the contraction of bladder tip as pacemaker cells.

Key words: Bladder, Interstitial cells of Cajal, Pacemaker cells

中图分类号:

R329.2

王江平, 王勤章, 丁国富. 豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义[J]. 中国临床解剖学杂志, 2014, 32(1): 45-47.

WANG Jiang-Beng, WANG Qi-Zhang, DING Guo-Fu. Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder[J]. Chinese Journal of Clinical Anatomy, 2014, 32(1): 45-47.

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豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义

Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder

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