豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义
Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder
目的 观察二聚体Cajal间质细胞(interstitial cells of Cajal，ICC)在豚鼠膀胱不同组织及部位的分布特点，并探讨其意义。 方法 电子显微镜下观察豚鼠膀胱壁组织切片黏膜层，黏膜下层，肌层内二聚体ICC分布情况；对膀胱组织切片进行免疫荧光染色，用c-Kit抗体标记ICC，激光共聚焦显微镜下观察二聚体ICC在膀胱顶部、体部、颈部的分布特点。 结果 在电子显微镜下见二聚体ICC主要分布于黏膜下层，而肌层主要以单体ICC为主。免疫荧光染色发现每高倍镜视野下膀胱顶部二聚体ICC平均数量为（3.47±0.53）个，体部和颈部为（1.57±0.45）个和(0.49±0.19)个。膀胱顶部二聚体ICC数量明显高于体部和颈部（P<0.01）。 结论 二聚体ICC主要分布于豚鼠膀胱顶部的黏膜下层，可能是感受黏膜张力刺激，引发顶部膀胱自发兴奋的起搏细胞。
Objective To observe the distribution of dimer interstitial cells of Cajal (ICC) in the different organizations and parts in guinea pig bladder. Methods The guinea pig bladder sections were made and the distribution of dimer ICC within different organizations were observed using electron microscope; The cryostat tissue sections of guinea pig bladder were made and dimer ICC marked with c-Kit antibody in different bladder parts were observed with immunofluorescence. Results Dimmer ICC, under electron microscope, was mainly distributed in the submucosa of detrusor while monomer ICC was mainly distributed between detrusor bundles. Under high-powered fields of confocal microscopy, the number of dimer ICC in bladder tip was 3.47±0.53 , bladder body was 1.57±0.45 and bladder neck was 0.49±0.19. Dimer ICC density in the bladder tip was significantly higher than that in the bladder body and neck (P <0.01). Conclusion Dimer ICC, mainly located in the submucosa of bladder tip, might accept the tension stimulation from mucous and then trigger the contraction of bladder tip as pacemaker cells.
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