Chinese Journal of Clinical Anatomy >
Behavioral evaluation and changes in levels of monoamine neurotransmitter within the nigrostriatal pathway in rat models of Parkinson's disease
Received date: 2014-09-01
Online published: 2015-04-21
Objective To study the changes in levels of monoamine neurotransmitter within the nigrostriatal pathway and evaluate the behavioral changes in rat models of Parkinson's disease. Method The unilateral rat models of PD were established by injecting 6-OHDA into the right medial forebrain bundle of adult Sprague-Dawley rats. After establishing the successful rat models, we observed their abnormal behavior and evaluated the behavioral changes (elevated body swing test, average velocity, actuation time and time length of rotation behavior). Additionally, we assayed the levels of monoamine neurotransmitter in the nigrostriatal pathway of PD rats by high performance liquid chromatography. Results (1) PD rats demonstrated some abnormal behavior such as stiffness of tail, akinesia or bradykinesia, tremor and the instable posture and gait, etc. (2) Statistically, elevated body swing test was not significant for the evaluation of PD rat models, while average velocity, actuation time and time length of rotation behavior were significant for the evaluation and showed a time-dependent pattern. (3) Both of the content of DA and serotonin were reduced in the substantia nigra and the striatum of the pathological lateral in PD rats, and the DA content decreased with time; Moreover, the degree of decline in DA content of the substantia nigra were respectively 62.96%, 82.88%, 92.71% and 91.50% and the striatum were respectively 77.47%, 91.39%, 96.25% and 96.18% during the second week, third week, fourth week and the fifth week. The average degree of decline in DA content of the substantia nigra was (89.03±5.36)% during the third week, fourth week and the fifth week by statistical analysis, while the striatum was (93.61±2.79)%. Conclusions (1) PD rats have the human-like clinical motor symptoms of PD, and the classic behavioral evaluation index of rotational behavior is average velocity, while actuation time and time length of rotation behavior play a supporting role in behavior evaluation of PD rat models; (2) There is a decline in the content of DA and serotonin of the substantia nigra and the striatum in the lesioned lateral of PD rats.
LIU Xiao-Jun , XIE Zhi-Ying , FU Xing-Hui , DI Mo-Qiang , JIA Chun-Lin . Behavioral evaluation and changes in levels of monoamine neurotransmitter within the nigrostriatal pathway in rat models of Parkinson's disease[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(2) : 182 -188 . DOI: 10.13418/j.issn.1001-165x.2015
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