Chinese Journal of Clinical Anatomy >
Effects of ketobodies metabolism on neuroprotection after acute spinal cord injury in rats
Received date: 2014-09-15
Online published: 2015-04-21
Objective Previous studies supported neuroprotective functionof ketogenic diet (KD) after spinal cord injury. The present study was aimed to investigate effects of pre-existing ketobodies metabolism on antioxidant and anti-apoptotic after acute spinal cord in rats. Methods Forty-eight male Sprague-Dawley rats were randomly assigned into 2 groups fed either with KD or standard diet (SD) for up to 2 weeks. Levels of blood ketones was measured for each rat at 2 weeks. All rats were subjected to a 1.5 mm contusion injury at 500 mm/s on the C5 spinal cord using an electromagnetic-servo material testing machine. Samples of spinal cord tissues were harvested at 3 h and 24 h after injury. Protein expression of Bax, caspase-3, Bcl-2 and CD68, indicating apoptosis and inflammation, were tested using double immunofluorescence. Contents of superoxide dismutase (SOD) and malondialdehyde (MDA), reflecting oxidative stress, were measured using ELISA.Results The blood ketone level in the KD group was 1.32 mmol/L, which was significantly higher than that in the SD group（0.38 mmol/L）（P<0.05）. Expression of Bax, Bcl-2,caspase-3 and CD68 were positive after injury. A significant difference in expression between groups was seen at 3 h and 24 h after injury. The content of SOD in the KD group was higher than that in the SD group at 3 h after injury, and there was a significant difference between groups at 24 h (540 pg/mL vs 281 pg/ml) (P<0.05). Change of levels of MDA was opposite to the change of SOD. The level of MDA in the KD group was 509 pg/ml, which was significantly lower than the level in the SD group (855 pg/ml) (P<0.05). Conclusion The present results indicated that a pre-existing high level of blood ketones can reduce oxidative stress and apoptosis in the spinal cord after acute contusion injury, suggesting neuroprotective function for acute spinal cord injury under ketobodies metabolism.
Key words： Ketogenic diet; SCI; Oxidative stress; Apoptioss; Neuroprotection
WANG Xiao-Meng , LIU Qi , ZHOU Jian , KONG Gang-Gang , TUN Xiu-Hua , HUANG Zhi-Beng , SHU Jing-An . Effects of ketobodies metabolism on neuroprotection after acute spinal cord injury in rats[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(2) : 176 -181 . DOI: 10.13418/j.issn.1001-165x.2015
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