Chinese Journal of Clinical Anatomy >
Proteomic study on influence of short-term binocular visual deprivation on the rat retina
Received date: 2014-03-24
Online published: 2015-02-12
Objective To explore the influence of visual deprivation on retinal protein expression. Methods The visual deprivation model is established by bilateral eyelid closure. Bilateral eyelid closure was performed at the postnatal 14 days, 2 weeks after which the rats were used for further experiment Two-dimensional gel electrophoresis was used to separate proteins in the retina, the differential proteins were chosen using PDQuest software and identified by tandem MS. the change of protein expression was further confirmed by Western blot and immunohistochemistry. Hematoxylin and eosin staining was performed to observe the retinal structure. Results Expressions of four proteins in the rat retina were decreased after visual deprivation. These proteins were identified as heat shock 70、alpha-enolase、serotranfferin and recoverin. However, we did not observe the change of the amoun of the retinal cells under the light microscope. Conclusions Short-term visual deprivation induces the change of retinal proteins, and may contribute to the occurrence of amblyopia.
Key words： Visual deprivation; Retina; Proteomics
HE Wen-Ji , LIU Jia-Li , ZHENG Fa-Wu , QI Bao-Gui , BO San-Jiang . Proteomic study on influence of short-term binocular visual deprivation on the rat retina[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(1) : 76 -80 . DOI: 10.13418/j.issn.1001-165X.2015
[1] Nataraj K, Le Roux N, Nahmani M, et al. Visual deprivation suppresses L5 pyramidal neuron excitability by preventing the induction of intrinsic plasticity
[J]. Neuron, 2010, 68(4):750-762.
[2] Duffy KR, Slusar JE. Monocular deprivation provokes alteration of the neuronal cytoskeleton in developing cat lateral geniculate nucleus
[J]. Vis Neurosci, 2009, 26(3):319-328.
[3] Hendrickson A, Boothe R. Morphology of the retina and dorsal lateral geniculate nucleus in dark-reared monkeys (Macaca nemestrina)
[J]. Vision Res,1976, 16(5):517-521.
[4] Feldkaemper M, Schaeffel F. An updated view on the role of dopamine in myopia
[J]. Exp Eye Res, 2013,114:106-119.
[5] Schmid KL,Rayner CL,Brown B. Hemi-field and full-field form-deprivation induce timing changes in multifocal ERG responses in chick
[J]. Ophthalmic Physiol Opt, 2013, 33(3):257-266.
[6] Kayama M, Nakazawa T, Thanos A, et al. Heat shock protein 70 (HSP70) is critical for the photoreceptor stress response after retinal detachment via modulating anti-apoptotic Akt kinase
[J]. Am J Pathol, 2011,178(3):1080-1091.
[7] Smith WC, Bolch S, Dugger DR, Li J, Esquenazi I, Arendt A, Benzenhafer D, McDowell JH. Interaction of arrestin with enolase1 in photoreceptors
[J]. Invest Ophthalmol Vis Sci, 2011, 52(3):1832-1840.
[8] Simons K. Amblyopia characterization, treatment, and prophylaxis
[J]. Surv Ophthalmol, 2005, 50(2):123-166.
[9] Makino CL, Dodd RL, Chen J, et al. Recoverin regulates light-dependent phosphodiesterase activity in retinal rods
[J]. J Gen Physiol, 2004,123(6):729-741.
[10]Repka MX, Kraker RT, Tamkins SM, et al. Retinal nerve fiber layer thickness in amblyopic eyes
[J]. Am J Ophthalmol, 2009, 148(1):143-147.
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