Chinese Journal of Clinical Anatomy >
The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells
Received date: 2014-04-27
Online published: 2015-02-12
Objective To study the expression of NGF and Laminin in RSC96 cells under different topography of eletrospun fibrous scaffolds. Methods According to different culture conditions, cultured RSC96 were divided into three groups, group A(aligned), group B(random) and group C(film), and all the three groups were cultured for three days. The total RNA of cells was extracted by Trizol method. Reverse transcriptase Oligo (dT) was used to synthesize cDNA. Then RNA was expanded by designed primer and used for q-PCR to detect the expression of NGF and Laminin. Results NGF mRNA expression of RSC96 was lower in group A , compared to group B and C (P＜0.05). There was statistical differences between group B and group C (P＜0.05). The laminin mRNA was expressed in all groups, but an decrease from group A to group C could be detected (P＜0.05). Conclusions The different expression of NGF and Laminin of RSC96 cells was induced by different topography of electrospun fibers and RSC96 cells may be directed towards a more mature state by aligned electrospun fibers.
Key words： RSC96 cells; NGF; Laminin; Electrospun fibers
CAO Xin , DIAO Li , ZHANG Xian-Zuo , QIN Jian-Jiang , LI Gong , TUN Gang . The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(1) : 60 -63 . DOI: 10.13418/j.issn.1001-165x.2015
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