Chinese Journal of Clinical Anatomy >
The study of the composite conduit containing nerve growth factor and alginate gel-polylactic acid for repair of facial nerve injury in rabbit
Received date: 2013-12-20
Online published: 2014-04-21
Objective To observe the effect of the composite conduit containing the nerve growth factor (NGF) and alginate gel-polylactic acid for repair of facial nerve injury in the rabbit. Methods Firstly, rabbit facial nerve injury model was established. The facial nerve stump was implanted into the prepared composite conduit containing NGF and alginate gel-polylactic acid for 2 mm, to keep the two facial nerve stumps for 10 mm. Then, the two ends of the catheter with nerve and were fixedand the skin sutured. The 12 rabbits were randomly divided into 3 groups: 4, 8 or 12 weeks after operation. After the experiment, the anastomotic site of facial nerve was cut and near, middle and distal tissue of the nerve conduit were selected for HE staining. Results The experimental rabbits showedprimary surgical wound healing, with no local necrosis, graft discharge or systemic adverse reactions observed. There was macrophage infiltration in catheter wall 4 weeks after operation; meanwhile, there were rich Schwann cells, fibroblasts and new small blood vessels in catheter proximal in this group. For 8 weeks after operation, fibroblasts and macrophages attaching to the catheter wallwas thinner than 4 weeks. Moreover, there was nerve bundle-like structure and separated fiber bundles within the conduit. The outer fibrous capsule was thickened and the small blood vessels reduced. For 12 weeks after operation, the nerve conduit wall was further decomposed associated with collagen fibers hyperplasia. The intraductal nerve fiber was close to normal, which showed thickening axon-like structure and thinning outer fibrous capsule. Conclusion The composite conduit containing NGF and alginate gel-polylactic acid shows good histo-compatibility and can effectively induce the facial nerve regeneration.
Key words： Nerve conduits; Facial nerve injury; Nerve growth factor; Alginate
DUAN Yong-Chang , TIAN An-Yong , ZHENG Wei , ZHANG Shan-Shan . The study of the composite conduit containing nerve growth factor and alginate gel-polylactic acid for repair of facial nerve injury in rabbit[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 189 -191 . DOI: 10.13418/j.issn.1001-165x.2014
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