Objective To compare the chondrogenic differentiation of human adipose mesenchymal stem cells (hASCs) in vitro by three components of chondrogenic induction medium. Methods hASCs were extracted from human adipose tissues and then performed in monolayer culture and microsphere culture respectively. Three kinds of different components of chondrogenic induction medium (group A: Fetal bovine serum (FBS) + high concentration TGF-β3, group B: Insulin, Transferrin, Selenium Solution (ITS) + low concentration TGF-β3, group C: ITS + high concentration TGF-β3) were added. After 3 weeks, the cell proteins in monolayer culture were extracted for detection of cartilage-related markers, and the cell microspheres were stained with Toluidine blue, Alcian blue, Sirius red to compare the effect of cartilage formation. Results After 3 weeks, the expression of cartilage markers was the highest in the group C of monolayer culture system, and the staining of microspheres was also the deepest in the group C. Conclusions As a medium additive, ITS has a better effect than FBS in inducing chondrogenic differentiation of hASCs. As an important growth factor stimulating chondrogenesis of hASCs, the concentration of 10 ng/ml TGF-β3 has a stronger effect than the concentration of 1 ng/ml.
Yu Qinghe, Qu Rongmei, Zhang Guowei, Li Xin, Qiu Xianshuai, Ouyang Jun, Dai Jingxing, Min Shaoxiong
. Comparison of chondrogenic effects of three kinds of chondrogenic induction media on human adipose-derived stem cells in monolayer and microsphere cultured[J]. Chinese Journal of Clinical Anatomy, 2021
, 39(1)
: 71
-75
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DOI: 10.13418/j.issn.1001-165x.2021.01.014
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