The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager&nbsp;

doi:10.13418/j.issn.1001-165x.2020.05.017

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (5) : 578-582. DOI: 10.13418/j.issn.1001-165x.2020.05.017

The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager

LI Shu-ting¹，ZONG Li-li¹，HE Wei-feng²，RAO Jun-hua³，LI Bi-hai³，LIU Yi-yan³，LIU Yu-jie¹，WANG Ting-wei⁴， LIU Ling-zi¹，WU Ting-feng⁵，WANG Bi-yun²，NIE Wei-zhe²

Author information +

History +

Abstract

Objective　To explore how to use PeriCam PSI blood perfusion speckle imager to detect the blood perfusion in tongues of cynomolgus monkey subjects and analyze its feasibility and application values.　Methods　1. Five female cynomolgus monkeys were subjected to cervical cerclage in preparation for their endometriosis (EMs) models. One of them only served as an indicator of pelvic conditions after its laparotomy. 2. PeriCam PSI blood flow perfusion speckle imager was used to measure the blood perfusion on the surfaces and bases of tongues of all cynomolgus monkeys for their preoperative conditions, in the very month of the coming surgery, and s.p. 1th, 2nd and 3rd months respectively. PIMSoft, the matched software, was used in data collection and analysis.　Results　1. Blood perfusion volume on tongues of the 6 subjects at different phases was successfully captured and five out of them s.p. cervical cerclage were seen to undergo significant ups/downs of their blood perfusion volume with statistical significance in their discrepancy. 2. The blood perfusion volume of tongue surfaces and bases of cynomolgus monkeys with s.p. 2 months mild-moderate pelvic adhesion was significantly higher than that of non-adhesion counterparts, the difference being statistically significant.　Conclusions　The method of PeriCam PSI blood perfusion speckle imager to detect the blood perfusion in tongues of cynomolgus monkey subjects is stably feasible and might be incorporated in one of the quantitative evaluation methods for modeled tongue pictures based on diseases-symptoms.

Key words

PeriCam PSI blood perfusion speckle imager / 　Tongue pictures / 　Blood perfusion volume；　Cynomolgus monkey model；　Animal surgery

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LI Shu-ting, ZONG Li-li, HE Wei-feng, RAO Jun-hua, LI Bi-hai, LIU Yi-yan, LIU Yu-jie, WANG Ting-wei, LIU Ling-zi, WU Ting-feng, WANG Bi-yun, NIE Wei-zhe. The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager [J]. Chinese Journal of Clinical Anatomy. 2020, 38(5): 578-582 https://doi.org/10.13418/j.issn.1001-165x.2020.05.017

References

[1] Humeau-Heurtier A, Mahé G, Abraham P. Microvascular blood flow monitoring with laser speckle contrast imaging using the generalized differences algorithm[J]. Microvascular Research, 2015,98:54-61.
[2] Eikermann-Haerter K, Lee J H, Yuzawa I, et al. Migraine mutations increase stroke vulnerability by facilitating ischemic depolarizations[J]. Circulation, 2012,125(2):335-345.
[3] Khan MB, Hoda MN, Vaibhav K, et al. Remote Ischemic Postconditioning: Harnessing Endogenous Protection in a Murine Model of Vascular Cognitive Impairment[J]. Translational Stroke Research, 2015,6(1):69-77.

[4] 宗利丽, 李亚里, 汪龙霞, 等. 子宫内膜异位症猕猴动物模型的建立[J]. 第一军医大学学报, 2003(10):1006-1009.

[5] Briers D, Duncan DD, Hirst E, et al. Laser speckle contrast imaging: theoretical and practical limitations[J]. Journal of biomedical optics, 2013, 18(6): 66018.

[6] Xie H, Zhang Y. Relationship between dynamic infrared thermal images and blood perfusion rate of the tongue in anaemia patients[J]. Infrared Physics & Technology, 2018, 89:27-34.
[7] 区绮云. 舌下络脉诊法诊查子宫内膜异位症的研究[D]. 广州中医药大学, 2008.
[8] Scheuzger JD, Zehnder A, Yeginsoy D, et al. Sublingual microcirculation：a case report[J]. Journal of Medical Case Reports, 2019,13(1).
[9] Imudia A, Kumar S, Saed G, et al. Pathogenesis of Intra-abdominal and Pelvic Adhesion Development[J]. Seminars in Reproductive Medicine, 2008, 26(4): 289-297.
[10]冯书梅. 慢盆逐瘀汤治疗慢性盆腔炎临床疗效及动物实验研究[D]. 广州中医药大学, 2011.
[11]冯晓冰. 剖宫产后盆腔粘连患者血清粘连相关指标与血流变指标的变化研究[J]. 中国医药科学, 2016, 6(22): 80-82.
[12]Zhang Y, Zhao L, Li J, et al. Microcirculation evaluation of facial nerve palsy using laser speckle contrast imaging: a prospective study[J]. European Archives of Oto-Rhino-Laryngology, 2019, 276(3): 685-692.
[13]Hoda MN, Fagan SC, Khan MB, et al. A 2 × 2 factorial design for the combination therapy of minocycline and remote ischemic perconditioning: efficacy in a preclinical trial in murine thromboembolic stroke model[J]. Experimental & Translational Stroke Medicine, 2014,6(1):10.