Protection and mechanism of isoflurane preconditioning against oxygen-glucose deprivation neuronal injury
ZHOU Guo-Bin, JI Jin-Quan, WANG Zhi-Feng, SUN Ke
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (6) : 702-705.
Protection and mechanism of isoflurane preconditioning against oxygen-glucose deprivation neuronal injury
Objective To study the protection and mechanism of isoflurane preconditioning against oxygen-glucose deprivation(OGD) neuronal injury. Methods Cortical neurons from rats at embryonic day 16 to 17 were cultured. With simuation of a cerebral ischemia in vitro, OGD was performed in the pretreated cells. Neuron injury was assessed by MTT. Protein expressionof pERK and HIF-1α was determined by Western blot. Results Isoflurane preconditioning protected dose-dependently the cortical neuron from OGD injury. The hypoxia inducible factor (HIF-1α) in the OGD injured neurons was increased by isoflurane preconditioning, which was partially abolished by ERK inhibitor PD98059. Conclusions Isoflurane preconditioning protects the cortical neuron from OGD injury, possibly through activating HIF-1α mediated by pERK.
Isoflurane preconditioning / Oxygen-glucose deprivation / Cortical neurons
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