Experimental animal model of pedicled diaphragmatic muscle flap for reconstruction of defected esophagus on pigs
FAN Xi-Ting, WANG Wu-Jun, WANG Xue-Lian, LIU Ta-Sheng, WANG Hao-Fei
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (5) : 568-571.
Experimental animal model of pedicled diaphragmatic muscle flap for reconstruction of defected esophagus on pigs
Objective This study aims to establish an animal model of diaphragmatic muscle flap with vascular pedicle for reconstruction of the esophagi in pigs, and compare the feasibilities, advantages and disadvantageso of using diaphragmatic muscle flaps with different vascular pedicles for reconstruction of the esophagusso as to lay an experimental foundation for further study. Method 10 healthy Landrace, weighing between 24 and 25 kg, were divided randomly into two groups (A and B) according to different vascular pedicles. Group A (n = 5), left phrenic artery was used as the vascular pedicle. Group B(n = 5), left pericardium phrenic artery with left phrenic nerve was used as thevascular pedicle. The lower 3~5 cm segment of the esophagi of the pigs in the two groups were cut off under general anesthesia, and diaphragmatic muscle flaps with vascular pedicles were harvested and rendered into tubes to reconstruct the defected esophagi. The then defected diaphragmatic muscles were repaired by dacron patch. Result The survival time and the cases of long-term survival animals in group B were greater than group A (P < 0.05); the isolated length of pericardium diaphragmatic arteries in group B was significantly greater than the isolated length of phrenic arteries in group A (P = 0.004); the length and width of diaphragmatic valves in Group B were greater than the feasible length (P =0.001) and width (P<0.001) in group A. Conclusion The experimental animal model of pedicled diaphragmatic muscle flaps reconstructing defected esophagi on pigs has been successfully established. It is feasible for diaphragmatic muscle flaps with pericardium phrenic artery or phrenic artery to reconstruct esophagi; the model of group A has more advantages than group B.
Esophagus / Diaphragmatic muscle flap with vascular pedicle / Arteria pericardiacophrenica / Inferior phrenic artery / Esophageal reconstruction
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