Expression of Thymidine kinase 1 (TK1) in osteosarcoma and its clinico-pathological significance
HU Mei-Quan, WANG Da-Beng, XIONG Jian-Xi, GUAN Hong, TUN Dun-Hui, HUANG Yu-Hua, SHU Wei-Min, LI Wei, YANG Ya-Ji, ZHANG Ji-Jun
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (5) : 564-567.
Expression of Thymidine kinase 1 (TK1) in osteosarcoma and its clinico-pathological significance
Objective To investigate the expression ofTK1 in osteosarcoma tumor cells and the TK1 level of TK1 in the serum of patients with osteosarcoma, and analyze their clinico-pathological significances. Methods 40 cases of osteosarcoma and 20 cases of giant cell tumor were included in this study. Immunohistochemical staining of TK1 was performed on the tumor tissue by MaxVision method. Serum TK1 level in 20 patients with osteosarcoma and 20 patients with bone trauma was tested by chemiluminescence dot blot enzyme chemistry. Results The reactivity assessed was in cytoplasm and nuclei. The positive rate of TK1 in high grade osteosarcoma was significantly higher than that in low grade osteasarcoma(P<0.05); The positive rate of TK1 in osteasarcomais was higher than that in giant cell tumor, and the difference was statistically significant(P<0.05). In addition, the serum TK1 content in patients with osteosarcoma is significantly higher than that in patients with bone traumatic [2.95 (0.97-7.25) pmol/Lvs 0.89 (0.32-2.58) pmol/L,P<0.05]. Conclusion TK1 has a certain value in the differential diagnosis of osteosarcoma from benign bone lesions. High level of TK1 can be tested in the serum in patient with osteosarcoma, and it seems that TK1 is a sensitive indicator for testing osteosarcoma proliferation.
Thymidine kinase 1 / Osteosarcoma / Immunohistochemical / Chemiluminescence
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