Effects of ginsenoside Rg1 on cell death model in brain tissue after cerebral ischemia-reperfusion in rats
XU Li, LIU Xia, BAO Cui-Fen, JI Gong-Chao
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (5) : 555-559.
Effects of ginsenoside Rg1 on cell death model in brain tissue after cerebral ischemia-reperfusion in rats
Objective To study the mechanism of ginsenoside Rg1 after cerebral ischemia-reperfusion in adult rats. Methods Rats were randomly divided into the sham-operative group（sham group）, Model group, ginsenoside Rg1 10、20、40 mg/kg groups, Nimodipine group（drug control group）. Cerebral ischemia-reperfusion(CIR) model was made by occluding the middle cerebral artery in rats. After CIR, the neurological deficit score was evaluated and IHC and Western blot technique was used to observe the expressions of PARP-1 and caspase-3． Results Compared with Model group, the neurological deficit scores and the expressions of PARP-1 and caspase-3 were significantly decreased. Conclusion Ginsenoside Rg1 can protect the brain cells from damage after CIR injury by down-regulating the expression of PARP-1 and caspase-3.
Ginsenoside Rg1 / Cerebral ischemia-reperfusion / Poly ADP-ribose polymerase-1 / Caspase-3
[1] Wyllie AH. Apoptosis: cell death in tissue regulation
[J].J Pathol,1987,153(4): 313-316.
[2] Chu X, Fu X, Zou L, et al. Oncosis, the possible cell death pathway in astrocytes after focal cerebral ischemia
[J]. Brain Res, 2007,29(1149):157-164.
[3] Shimizu S. Apoptosis and non-apoptotic cell death
[J].Rinsho Ketsueki,2011, 52(10):1669-1676.
[4] 李朝，邵姝元，郭敏，等.小鼠胚胎肾细胞凋亡与胀亡的超微结构
[J].解剖学杂志，2011，34（4）：484-486.
[5] Wang LB, Zhang LY, Shan CH.A new form of cell death: Parthanatos
[J].Yi Chuan,2010, 32(9):881-885.
[6] 辜清, 万勇, 吴娣, 等.大鼠脑缺血再灌注后运动皮质细胞凋亡的体视学观察
[J]. 南昌大学学报(理科版), 2009,33(1): 68-72.
[7] Sayk F, Bartels C.Oncosis rather than apoptosis
[J]? Ann Thorac Surg,2004,77(1): 382-383.
[8] Castejón OJ, Arismendi GJ. Nerve cell death types in the edematous human cerebral cortex
[J].J Submicrosc Cytol Pathol,2006,38(1):21-36.
[9] Koh DW, Dawson TM, Dawson VL. Mediation of cell death by poly(ADP-ribose) polymerase-1
[J]. Pharmacological research, 2005, 52(1):5-14.
[10] 贾红燕,李辉宇,赵浩亮.人参皂苷Rg1注射液对犬肝脏缺血再灌注损伤的保护作用
[J].山西医科大学学报，2010, 41(1)：8-10.
[11]Zhang X, Wang J,Xing Y,et al.Effects of ginsenoside Rg1 or 17β-estradiol on a cognitively impaired, ovariectomized rat model of Alzheimer's disease
[J]. Neuroscience,2012,18(220):191-200.
[12]Huang T,Fang F,Chen L,et al.Ginsenoside Rg1 attenuates oligomeric Aβ(1-42) induced mitochondrial dysfunction
[J].Curr Alzheimer Res,2012,9(3): 388-395.
[13] 李源莉.人参皂甙Rg1对脑缺血再灌注致小鼠皮质神经元损伤的保护作用及其机制探讨
[D].西安:第四军医大学,2007.
[14]包翠芬.人参皂苷Rg1对抗脑缺血再灌注细胞凋亡及p-JNK表达的实验研究
[D].锦州:辽宁中医药大学,2009.
[15]刘霞.人参皂苷Rg1对抗脑缺血再灌注ERK通路的实验研究
[D].锦州:辽宁中医药大学,2010.
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