Objective　To provide anatomical and morphologic basis for facial rejuvenation.  Method　6 cases (12 sides) adult cephalic specimens were anatomized.  The landmarks of the glabella muscles, including the corrugator, the depressor supercilii and the procerus were measured. The weights of the muscle were also examined and photographed. CT scan data of heads were obtained after human intervention, and 3D model was set up, demonstrating glabella muscles clearly.　Results　The clinical anatomy clearly showed the anatomical basis of glabella muscles,  the glabella muscles included corrugator and glabella depressor.  Corrugator could be divided into two broad categories: transverse and oblique, which could be generally recognized from the morphology of the eyebrows. Among them, the oblique type could be divided into oblique single beam type and oblique beam type according to its shape. the glabella depressor included depressor supercilii and Procerus. There was individualized morphology and development of the glabella depressor.　Conclusions　 The glabella muscles are the potent targets for the use of botox injection. Through exploration of the anatomical and morphologic characteristics of the glabella muscles, measurement of related data, 3d model can be easily set up, which can demonstrate the glabella clearly. It is of great clinical significance for customizing the use of Botulinum Toxin Injection.