Objective　To provide basic data for the application of CTVE to transsphenoidal surgery. Methods　In fifteen injected adult cadaveric heads, the CTVE images of sphenoid sinus were reconstructed by Navigator Guide software, and then compared with the endoscopic images.　Results　CTVE and endoscope could display the superficial anatomic landmarks such as the sphenoid ostia, optic prominence, carotid prominence, opticocarotic recess and clival indentation of sphenoid sinus in a three-dimensional mode. The difference between the display rates of CTVE and endoscope are not statistically significant（P>0.05）. CTVE could display all the anatomies of the underlying carotid ateries by Transfer Functions. By gradual heightening of the threshold, CTVE could display the underyling optic canals and simulate the procedures of sellar floor exposure and optic nerve decompression, which were similar to the endoscope.　Conclusion　CTVE can display the superficial and underlying anatomies involved in transsphenoidal surgery in a three-dimentional mode. CTVE can be a valuble imaging tool for transsphenoidal sugery.