The effects of macrophage in different activation states on NGF and Laminin expressions of RSC96 cells
DIAO Li, CAO Xin, OU Yang-Jun, TU Lei, ZHANG Xian-Zuo, LIU Re-Yan, XUE Xu-Kai, LEI Zhi-Xian, QIN Jian-Jiang
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3) : 321-324.
The effects of macrophage in different activation states on NGF and Laminin expressions of RSC96 cells
Objective To study the expression of NGF and Laminin in RSC96 cells under co-culture with macrophages in different activation states. Methods A Transwell system was built to co-culture RSC96 cells with rat peritoneal macrophages. Real-time PCR, western blotting, and ELISA were used to detect NGF and LN expression in each group. Results Under macrophage co-culture, the NGF expression in RSC96 cells was increased. A significant promotion has been found with activated macrophages. With regard to LN expression, no significant difference was detected. Conclusions The macrophage could contribute to the NGF expression in RSC96 cells, the effect of which is influenced by the activation states. Activated macrophages showed greater effect. No such promotion is found on LN expression, which is associated more with the migration of the Schwann cells. Further research is needed.
RSC96 cells / Macrophages / NGF / Laminin / co-cultured
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