The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation
YAN An-Bin, TU Nan-Sheng, LEI Wei-Jie, LEI Yong-Hui, JIAN Dong-Yang
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3) : 314-317.
The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation
Objective To investigate the effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation. Methods LIM mineralization protein overexpression lentiviral vector was constructed and transfected into osteosarcoma cell line OS-9901 cells by liposome. Quantitative PCR and Western blot were performed to measure the relative mRNA and protein expression of LIM mineralization protein 1, and BrdU assay was performed to detect cell proliferation before and after the LMP-1 over-expression. Results LMP-1 mRNA and protein expression did not change significantly (P>0.05) at 6 h after cells transfected with the LMP-1 expression lentiviral vector. When LMP-1 mRNA and protein expression were significantly increased at 12h, 24h and 48h after cells transfected with the LMP-1 expression lentiviral vector, DNA synthesis was significantly lower (P<0.05). Conclusion LIM mineralization protein 1 overexpression can inhibit osteosarcoma cell line OS-9901 cell proliferation.
LIM mineralization protein 1 / Osteosarcoma cells / Lentiviral vector
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