The influence of down-regulation of TAp73 expression by short hairpin RNA on the proliferation and invasion of osteosarcoma
XUE Jian, JIN An-Min, LV Hai, MIN Shao-Xiong
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (2) : 170-173.
The influence of down-regulation of TAp73 expression by short hairpin RNA on the proliferation and invasion of osteosarcoma
Objective To investigate the pro-apoptosis, anti-migration and anti-proliferation of Saos-2 cells in osteosarcoma through genetic inhibition of expression of TAp73 protein. Methods Human osteosarcoma cell line Saos-2 was cultured．A short hairpin RNA targeting TAp73 was constructed and transfected into Saos-2 cell line．The expression of TAp73 protein were examined by Western blot method．The proliferation was assayed by CCK-8．Flow cytometry was used to detect the cell cycle．Transwell test was used to detect the invasion ability of Saos-2. Results Western blot revealed notably down-regulated expression at protein levels of Tap 73．The cellular proliferation in the treatment group detected by CCK-8 was active after siRNAtreatment(P<0.05)．The proportion of the cells in the S phase increased．The invasion ability increased from（ 84.17±1.35） to (92.67±2.80 )(P<0.01)． Conclusions TAp73 is necessary for human osteosarcoma cell proliferation and invasion．It is probably a potential target for osteosareoma treatment．
Osteosarcoma / Tap73 / SiRNA / Proliferation / Invasion
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