Alteration of myelin basic protein expression and nerve fiber degeneration after compression injury of rat spinal cord
WU Xiu-Yu, SUN Shan-Quan, ZHONG Xiao-Yan, ZHANG Bei, HONG Ke-Jian, HUANG Sai-Qin
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (6) : 658-662.
Alteration of myelin basic protein expression and nerve fiber degeneration after compression injury of rat spinal cord
Objective To evaluate the changes of nerve fiber degeneration and myelin basic protein(MBP) expression in rat white matter after the compression spinal cord injury(CSCI). Methods Eighty-four adult Sprague-Dawley rats were randomly divided into 3 groups: normal group（n=12）, operation group（n=36） and the sham group（n=36）. The CSCI model was established with a self-made device for animals of operation group. The operation process in the sham group was the same as the operation group except for the compression of spinal cord. The segments of compressed spinal cord were properly taken at an interval of 1, 3 and 7 day after operation. Luxol fast blue(LFB)， immunofluorescence (IF) staining and western blot were performed to observe the pathological changes of white matters. Results LFB staining showed that after compression the degeneration of nerve fiber occurred in the white matter and a great number of fibers were lost. Compared with the normal and sham operation groups, the loss of myelinated fibers was markedly decreased in compressed segments of the model group (P<0.05). IF showed that MBP- positive fibers were degenerated and lost, coinciding with LFB staining. The expression of MBP was progressively significantly declined after compression. Conclusions Demyelination occurs, followed by the time-dependently reduced expression of MBP in white matter of model animals. Expression decrease of MBP may be related to the demyelination.
Spinal cord compression / Nerve degeneration / Demyelination / Myelin basic protein / Rat
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