The effect of human umbilical cord plasma on the growth of human foreskin fibroblast
DING Yan, LEI Zhi-Yong, YUAN Ya-Gong, MENG Chu-Yan, FENG Jing-Bei, LI Dong-Sheng, CENG Yi
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (5) : 548-551.
The effect of human umbilical cord plasma on the growth of human foreskin fibroblast
Objective To investigate whether human umbilical cord plasma (HUP) can be used to culture human foreskin fibroblast (HFF) cell. Methods Surplus HUP were collected in this study. After being treated by salting out and diasysis, the HUP were used to culture HFF as 10% volume, and compared the effects with FBS. The features, growth curve, cell cycle and reproductive activity of HFF were observed. Results compared to fetal bovine serum (FBS), the morphous, reproductive activity and fibroblast characteristic of HFF cell cultured in HUP were not distinctively different. Conclusions HUP can be used to culture HFF.
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