Complex of collagen sponge seeded rat amniotic epithelial cells promotes survival and revascularization of rat prefabricated flap
WANG Ying-Qian, WANG De-Wei, HU Jia-Jun, LI Ling-Qiao, ZHOU Gong, WEI Zhen-Bang, WANG Tian-Hao
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (5) : 533-538.
Complex of collagen sponge seeded rat amniotic epithelial cells promotes survival and revascularization of rat prefabricated flap
Objective To investigate the effects of amniotic epithelial cells/collagen sponge complex on promoting survival of rat prefabricated flap. Methods The 3rd passage of SD rat amniotic epithelial cells (AECs) or chondrocyte line was seeded into the collagen sponge scaffold. ELISA was taken to detect angiogenesis factors VEGF, TGFβ1, bFGF in the supernatant fluid of the AECs complex. SD rats were divided into normal control group, blank control group, chondrocyte group and AECs group. Angiogenesis factors in the flaps of each group were detected at the day 7 and 14 after the first-stage surgery. The survival of the flaps and microvasculature density were compared and evaluated by HE staining, vWF immunohistochemistry and digital image analysis system respectively one week after the second-stage surgery. Results The level of VEGF, TGFβ1 and bFGF in the supernatant fluid of the AECs complex increased gradually, and the highest concentration was detected in flap tissue of AECs group. The survival rate of the flap in the AECs group was higher than that in the blank control group and chondrocyte group. Both subcutaneous microvascular area ratio and vWF positive area ratio in the AECs group were higher than those in the other groups. Conclusions AECs/collagen sponge complex can increase the survival of prefabricated flap evidently. Angiogenesis factors secreted by the AECs promote the revascularization in the prefabricated flap.
Amniotic epithelial cells / Collagen sponge / Prefabricated flap / Revascularization / Angiogenesis factors
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