Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro
ZHONG De-Jun, LI Sen, KANG Min, XU Shuang, WEI Shu-Yi, WANG Qing, WANG Song
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (4) : 426-430.
Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro
Objective To explore the expression and effect of BMP-2 during the rat embryonic stem cells(NSCs) induced by all-trans-retinoic acid（ATRA）differentiating into neurons in vitro. Methods The rat embryonic neural stem cells were differentially induced with 1.0 μmol/L ATRA in vitro. The expression of BMP-2 was respectively investigated by immunocytochemistry, real-time PCR and western blot at the time of inducing 3d, 5d, 7d and 9d, and compared with that of untreated cells in control group. Results The highest expression of BMP-2 was at the time of inducing 3d, and then it decreased from inducing 5d following the NSCs differentiating. There was no significant difference between 7d and 9d groups. Conclusions The expression of BMP-2 decreases in the progress of NSCS differentiating into neurons in vitro, which indicate that, the inhibition of BMP2 signal pathway participate the differentiation process of NSCs.
Neural stem cells / All-trans-retinoic acid / BMP-2 / Differentiation / Expression
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