Acute myoinjury induce the intramuscular infiltration of CD8+ CTL cells
FENG Li-Jiang, CENG Hui-Jun, LIU Nie-Hui, CHEN Rong, HUANG Wei-Yi, LIAO Hua
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (3) : 307-310.
Acute myoinjury induce the intramuscular infiltration of CD8+ CTL cells
Objective To investigate the intramuscular infiltration of CD8+ cytotoxic T-cells （CTL cells） after crush myoinjury. Methods Tibialis antierior muscle(TA) crush was performed in C57BL/6 mice. HE staining was used to identify inflammatory reaction，degeneration and regeneration of crushed TA muscle. Immunofluorecence was used to detect pro- and anti- inflammatory cytokines, and the intramuscular infiltration of CTLs in injuried muscle. Results Crush induced significant myofiber necrosis, degeneration and regeneration. Conspicuous mononuclear cell infiltrates were detected at both d4 and d7 after crush injury. At d7 post-injury CTLs expressing perforin or IFN-γ were conspicuously detected in crushed muscle where CD8+ T-cells were found in close contact with regenerating myofibers. Conclusions After crush injury, CD8+ T cells infiltrate the injured muscle at regenerating period, and are transiently activated as CTL cells.
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