The treatment of skin wound healing of rats by different transplantation approaches of BMSCs combination with Activin B
SUN Li, WANG Xue-Er, ZHANG Min, CHEN Shi-Xuan, KONG E-Nan, CUI Meng-Qing, ZHANG Lu, ZHANG Lin
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (2) : 197-202.
The treatment of skin wound healing of rats by different transplantation approaches of BMSCs combination with Activin B
Objective To discuss the optimum transplantation approaches of BMSCs combination with Activin B on skin wound healing. Methods BMSCs were identified by immunocytochemistry. Residual situation of the CFSE-labeled BMSCs after transplantation was detected by the In Vivo imaging system and frozen section. Wounded areas were recorded and wound healing was observed by the paraffin section and HE staining. Results Positive expression of CD90 and negative expression of CD80 were observed by immunocytochemistry. Fluorescence of the wounded sites of rats in the experimental groups was observed by In vivo imaging system, but it didn't be observed in control group at the 7 and 14 days after skin wounding. Wound healing rate in the experimental group was increased in comparison with that in the control group (P<0.05), but wound healing rate in the different transplantation approaches had no significant difference. Conclusions BMSCs transplantation via the epidermal and the tail vein can both migrate to the wounded sites and promote the wound healing. Compared with the tail vein transplantation group, wound repair in the epidermal transplantation group was more simple and feasible.
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