Differential expression of NPY in superior cervical ganglion of SHR rat
ZHANG Shi, HUI Chu-Ping, WANG Shi, XU Feng-Xiao
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (1) : 87-90.
Differential expression of NPY in superior cervical ganglion of SHR rat
Objective To explore the effects of neuropeptide Y (NPY) on the occurrence and development of hypertension by observing its expression differentiation in superior cervical ganglion of spontaneous hypertensive rat (SHR). Methods We randomly selected SHR (n=20) and WKY (n=20) and observed the location, the shape, the size and the weight of the superior cervical ganglion. Real-time PCR and immunohistochemical PV-9000 method were used to examine the mRNA and protein expression of NPY in superior cervical ganglion of SHR and WKY. Results：The blood pressure of SHR was obviously higher than that of the WKY group（P<0.05）. The results of Real-time PCR and the immunohistochemistry showed that the mRNA and protein expression of NPY in superior cervical ganglion of SHR increased obviously than those in WKY（P<0.05）. Conclusions NPY may participate in the occurrence of hypertension by some complex mechanisms.
SHR / NPY / Cervical ganglion / Real-time PCR
[1] Malmstrom RE. Pharmacology of neuropeptide Y receptor antagonists.Focus on cardiovascular functions
[J]. Eur J Pharmacol, 2002, 447(1):11-30.
[2] 刘培党,晋光荣,王鹤鸣,等.大鼠颈上交感神经节的形态学研究
[J].徐州医学院学报,2000,20(1):36-38.
[3] Hashimoto R, Udagawa J, Kagohashi Y. Direct and indirect effects of neuropeptide Y and neurotrophin 3 on myelination in the neonatal brains
[J]. Brain Res, 2011, 1373(1):55-66.
[4] Montecucco F, Pende A, Quercioli A. Inflammation in the pathophysiology of essential hypertension
[J].J Nephrol, 2011, 24(1):23-34.
[5] Grassi G. Sympathetic neural activity in hypertension and related diseases
[J].Am J Hypertens, 2010, 23(10):1052-1060.
[6] Alves FH, Crestani CC, Resstel LB. Cardiovascular effects of noradrenaline microinjected into the insular cortex of unanesthetized rats
[J]. Auton Neurosci, 2011,160(1-2):90-98.
[7] Dorn GW 2nd. Adrenergic signaling polymorphisms and their impact on cardiovascular disease
[J].Physiol Rev, 2010, 90(3):1013-1062.
[8] Zhao XH, Sun XY, Edvinsson L. Does the neuropeptide Y Y1 receptor contribute to blood pressure control in the spontaneously hypertensive rat
[J]. J Hypertens, 1997, 15(1):19-27.
[9] Kuo LE, Abe K, Zukowska Z. Stress, NPY and vascular remodeling: Implications for stress-related diseases
[J].Peptides, 2007, 28(2):435-440.
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