Effects of silicon on the activity of the nuclear transcription factor NF-kappa B in osteoblasts
QIU Xiao-Zhong, WANG Yong-Kuai, WANG Le-Yu, TU Lei, WANG Guo-Bao
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (1) : 84-86.
Effects of silicon on the activity of the nuclear transcription factor NF-kappa B in osteoblasts
Objective To explore the effects of silicon on the activity of nuclear transcription factor NF-kappa B in osteoblasts cultured in vitro. Methods MC3T3-E1 osteoablasts were processed by sodium silicate solution (SiO32-) with the final concentration of 1mM and 2 mM for 6h, 12h, 24h and 48h, respectively. Untreated cells were taken as the control groups. Flow cytometry was used to detect the cell cycle and calculate cell proliferation index. Western blotting was used to detect the expressions and changes of NF-kappa B signaling pathway related proteins. Results Compared with the controls, significant proliferation of MC3T3-E1 cells was found in the 24h and 48h groups treated with 1 mM silicon. Western blot analysis showed that the pro-proliferation effect of 1 mM silicon on osteoblasts was closely related to the high-expression of p-NF-kappa B. Conclusions Trace amounts of silicon released from bone materials can not cause damage of osteoblasts, on contrary, trace amounts of silicate may induce proliferation of osteoblasts by activating NF-kappa B signaling pathway.
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