The expression of integrin β1 in rat hypertrophic myocardium induced by isoprenaline
WU Dun-Fang, GUO Zhi-Kun, ZHANG Dong, GAO Jian-Hui
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (1) : 79-83.
The expression of integrin β1 in rat hypertrophic myocardium induced by isoprenaline
Objective To explore the expression changes of the integrin β1 in hypertrophic rat myocardium. Methods The rat models of myocardial hypertrophy were prepared by isoprenaline(ISO) injection into dorsal subcutis at 7d, 28d and 56d respectively. Myocardial hypertrophic indexes of model animals were assessed. Immunohistochemisty and western blot were used to investigate integrin β1 expression in left ventricular tissue. Results Integrin β1 presented mainly in myocardial membrane. The expression of integrinβ1 in hypertrophic myocardium was higher than that of control group（P<0.05）, furthermore, the expression level increased gradually from 7d, 28d to 56d. Conclusions Along the development of cardiac hypertrophy, the expression level of integrin β1 increases gradually, which demonstrates that integrin β1 plays an important role during the development of myocardial hypertrophy.
Integrin β1 / Immunohistochemistry / Western blot / Myocardial hypertrophy model / Rat
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