Objective　To investigate clinical values of preoperative and postoperative magnetic resonance spectroscopy (MRS) on the excision of glioma.　Methods　16 patients with glioma were checked by routine magnetic resonance imaging (MRI) and 3D-MRS before and after operation, calculated and analyzed the ratio of Cho/Cr and Cho/NAA respectively. The excision area was assessed according to the position and corresponding relation of the tumors. As well, specimen tissue around the 'tumor' was collected during the operation to make pathologic examinations, for confirming the excision degree and compared it to MRS results.　Results　Integrated MRS and pathologic data were collected from 11 cases. According to the pathologic standard, 3 cases were complete excision, 8 cases were partial excision. However, 3D-MRS presented 2 cases of complete excision, and 9 cases of partial excision. There was no significant difference between two way (P> 0.05).　Conclusions　 It is a new application of MRS for evaluating excision degree of glioma noninvasively.