The effects of Ang-1 on the angiogenesis and vascular leakage of diabetic rats' femoral head
CHEN Hai-Yang, WANG Xiu-Guo, CHEN Shao-Jiang, LIU Wen-Ge, TANG Jun-Min
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (6) : 681-685.
The effects of Ang-1 on the angiogenesis and vascular leakage of diabetic rats' femoral head
Objective To probe into the effects of Ang-1 on the angiogenesis and microvascular leakage of diabetic rats' femoral head. Methods By establishing models of the rats with STZ diabetes, and randomly dividing them into the normal groups (CON1, CON2 and CON3) and the diabetic groups (DM1, DM2 and DM3) with 10 rats each group, the microvascular density through infusion with ink, the expression of blood coagulation factorⅧ with immunohistochemistry, and the expression of the mRNA of both VEGF by hybridization in situ and Ang-1 by RT-PCR, were performed. Results The diabetic rats'femoral head varied in the course of illness. Their expression of Ang-1, blood coagulation factorⅧ, and VEGF mRNA significantly rose, as compared with those of rats in the normal groups(P<0.01). The microvascular density enlarged with vascular proliferation and leakage. Conclusions The interaction and antagonism between Ang-1 and VEGF mRNA of diabetic rats' femoral head can promote microvascular proliferation and resist vascular leakage. The mRNA expression of both coagulation factorⅧ and VEGF in vascular endothelial cells have a positive correlation with the changes of their microvascular density.
Diabetes / Ang-1 / Micrangium / Vascular endothelial growth factor / F-Ⅷ / Infusion with ink / Hybridization in Situ
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