The protection of salvia miltiorrhiza compostita to blood brain barrier on severe burn injuried rats
LIU Ceng-Xu, TU Qiang, WANG Xiang-Dong, ZHOU Cong-Fa, LI Yao-Bin, LIU De-Meng
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (5) : 565-567.
The protection of salvia miltiorrhiza compostita to blood brain barrier on severe burn injuried rats
Objective To explore the effects of salvia miltiorrhiza compositita on blood-brain barrier permeability and ZO-1 expression in burn injuried rats. Methods In this research, healthy Sprague-Dawley rats were separated into three groups as follows: sham scald group, scald group, scald and salvia miltiorrhiza group. Changes in BBB permeability were determined by detected Evans blue (EB) content in rats' brain with chemical method. Furthermore, in order to explore the molecular mechanism of BBB disruption in rats' brain after severe burn, the expression levels of ZO-1 mRNA was analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results Expression of brain ZO-1mRNA significantly decreased during 3 to 48h after burn. The contents of Evan's bluedye (EB) in brain significantly improved at the 3h and reached the highest level during 6 to 24h after burn injury. After treating with salvia miltiorrhiza, EB content in the brain of burned rats was corrected obviously, especially at the time points of 3, 6h after burn injury. As well, ZO-1mRNA in brain increased after drug treating, and reached the highest level at 3h after burn injury. Conclusions Salvia miltiorrhiza will prevent the decrease of ZO-1 level in brain after burn injury, which is helpful for alleviating the burn damage on blood-brain barrier.
Burn injury / Blood-brain barrier / Zonula occludens / Salvia miltiorrhiza compostita(SMC)
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