Inflammatory pain induced CBP expression change in rat hippocampus
LI Hua, CAO Wen-Yu, XU Yang, LUO Yan-Wei, ZHONG Xiao-Lin, ZHANG Jian-Wei, LI Chang-Qi, LUO Hua-Gang, DAI Ru-Ping
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (5) : 546-549.
Inflammatory pain induced CBP expression change in rat hippocampus
Objective To explore expression changes of CBP（CREB Binding Protein) in rat hippocampus followed by Complete Freund's Adjuvant(CFA) induced inflammatory pain. Methods Rats were received plantar subcutaneous injection of CFA and saline mixed solvent 100ul, for establishing inflammatory pain model. Model rats were randomly divided into normal group, CFA 6h, 1d, 3d, 7d and 14d groups. The change of paw withdrawal threshold (PWT) was observed by Von Frey fibers in rats at different time points; the expression of CBP was detected using immunohistochemistry. Results PWT decreased quickly at 1h after CFA injection, and to the lowest value at 6h. PWT gradually rose at 3d and was still lower than baseline at 14d. A number of CBP positive products can be seen in hippocampus. CBP was significantly decreased at 6h and 1d after CFA injection; and increased at 3d, then decreased again from 7d to 14d after CFA injection，especially in CA1 and DG. Conclusions CFA can induce inflammatory pain about 2 weeks. CBP in hippocampus shows biphasic expression following inflammatory pain，which indicate gene transcription change in hippocampus plays an important role in the induction and maintenance of persistent pain.
Inflammatory pain / Hippocampus / CBP / Gene transcription / Rat
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