The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat
WANG Qing-Zhi, ZHOU Li, WANG Zhi-Yong, GUO Zhi-Kun
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (3) : 321-324.
The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat
Objective To investigate the expression of HCN2 and HCN4 in the left ventricles of type 1 diabetes rat. Methods 60 rats were randomly divided into control group (N, n=20) and diabetes group (T1DM, n=40). For T1DM group, streptozotocin was injected into peritoneal cavity of the animals to establish the model of type 1 diabetes. At the 90d after injecting, the expression of HCN2 and HCN4 were detected by immunofluorescent staining, Western-blot and RT-PCR respectively. Results Immuno-fluorescent staining presented the discontinuous punctiform or linear expression of HCN2 in cardiomyocytes membrane, but not for HCN4. However, Both HCN2 and HCN4 were up-regulated at protein(western-blot) and RNA (RT-PCR) levels compared to that of controls. Conclusions The over expression of HCN2 and HCN4 maybe participate in the electrical remodeling in DM rats and the developing of ventricular arrhythmia.
HCN2 / HCN4 / Left ventricle / T1DM / Rat
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