In vitro biocompatibility of bone marrow mesenchymal stem cells and nano-hydroxyapatite scaffold
ZHANG Kai, WANG Da-Ping, ZHU Wei-Min, LIU Jian-Quan
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (2) : 213-216.
In vitro biocompatibility of bone marrow mesenchymal stem cells and nano-hydroxyapatite scaffold
Objective To assess the compatibility of rabbit bone marrow mesenchymal stem cells(rBMSCs) with nano-hydroxyapatite(nano-HA) scaffold，and to verify the feasibility of nano-HA material as scaffold material of bone tissue engineering. Methods rBMSCs were cultured on nano-HA scaffold in vitro. Inverted microscope and scanning electron microscope were used to observe the morphologic features of cultured cells attached on the scaffold, as well, cell proliferation and differentiation under the influence of the material were detected by MTT and ALP methods. Results On the nano-HA scaffold, rBMSCs attached and survived well. Scaffold did not interfere with the proliferation and differentiation of the seeded rBMSCs, which indicated that Nano-HA scaffold was favourable for the survival and differentiation of rBMSCs in vitro condition. Conclusions Nano-HA material can be taken as the ideal rBMSCs scaffold for its excellent biocompatibility.
Bone marrow mesenchymal stem cells(rBMSCs) / Nano-hydroxyapatite / Biocompatibility
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