The expression of SDF-1α in the malformation of cortical development model rats
ZHANG Yi, YAN Yong, ZHANG Jian-Gang, HUANG Min, HE Xin
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (1) : 72-76.
The expression of SDF-1α in the malformation of cortical development model rats
Objective To get a message of the expression about stromal cell-derived factor-1α（SDF-1α）in the Malformations of cortical development（MCD）in rats model and investigate the effects in forming abnormal neural circuit in the MCD. Methods Sprague-Dawley pregnant rats exposed to X-irradiation on embryonic 16（E16）days can induce MCD in the offspring. We observed the histological changes of the brain with HE staining and Timm staining，and the expression of SDF-1α with immunohistochemistry，RT-PCR and Western-Blot in the pups at postnatal 30 days（P30）and postnatal 60 days（P60）. Results ①Timm staining demonstrated mossy fiber sprouting was formed in the MCD model；②Immunohistochemical results showed that expression of SDF-1α appeared in the CA1 ectopic neurons of hippocampus；③From the results of RT-PCR and Western-Blot，we got a reduced expression of SDF-1α at P30 and P60 in the model group contrast to control group at the same period. Conclusion the downregulation of SDF-1α may play a role in forming abnormal neural circuit in hippocampus of MCD.
Malformations of cortical development / Stromal cell-derived factor-1α / / Mossy fiber sprouting / Epilepsy
[1] Pang T, Atefy R, Sheen V. Malformations of cortical development
[J]. Neurologist, 2008,14(3):181-191.
[2] Paredes MF,Li G,Berger O,et al.Stromal-derived factor-1 (CXCL12) regulates laminar position of Cajal-Retzius cells in normal and dysplastic brains
[J]. Neurosci, 2006, 26(37):9404-9412.
[3] Nakanishi K,Watanabe K, Kawabata M,et al.Altered synaptic activities in cultures of neocortical neurons from prenatally X-irradiated rats
[J]. Neuroscience Lett, 2004, 355(1-2):61-64.
[4] Shetty AK, Zaman V, Shetty GA. Hippocampal neurotrophin levels in a kainate model of temporal lobe epilepsy: a lack of correlation between brain-derived neurotrophic factor content and progression of aberrant dentate mossy fiber sprouting
[J]. Neurochem, 2003, 87(1):147-159.
[5] Miki T, Sawada K, Sun XZ, et al. Abnormal distribution of hippocampal mossy fibers in rats exposed to X-irradiation in utero
[J]. Brain Res Dev Brain Res, 1999,112(2):275-280.
[6] 马勋泰，晏 勇，晏宁,等.皮质发育障碍模型鼠脑皮质形态学和致痫机制研究
[J].中国临床解剖学杂志，2006，24（5）：541-545.
[7] 马勋泰，晏勇，王学峰. 液氮损伤诱导局灶性皮质发育不良模型鼠脑组织病理特征
[J].中国神经免疫学和神经病学杂志，2008，15（1）：39-42.
[8] Fan QY, Ramakrishna S, Marchi N, et al. Combined effects of prenatal inhibition of vasculogenesis and neurogenesis on rat brain development
[J]. Neurobiol Dis, 2008, 32(3):499-509.
[9] Janowski M.Functional diversity of SDF-1 splicing variants
[J].Cell Adh Migr, 2009, 3(3):243-249.
[10]Chalasani SH,Sabelko KA,Sunshine MJ,et al.A Chemokine,SDF-1,Reduces the Effectiveness of Multiple Axonal Repellents and Is Required for Normal Axon Pathfinding
[J].Neurosci, 2003, 23(4):1360-1371.
[11] Li M,Ransohoff RM.Multiple roles of chemokine CXCL12 in the central nervous system: a migration from immunology to neurobiology
[J].Prog Neurobiol, 2008, 84(2):116-131.
[12] Ohshima Y,Kubo T,Koyama R,et al.Regulation of Axonal Elongation and Pathfinding from the Entorhinal Cortex to the Dentate Gyrus in the Hippocampus by the Chemokine Stromal Cell-Derived Factor 1α
[J].Neurosci, 2008, 28(33): 8344-8353.
[13] Li G,Kataoka H,Coughlin SR,et al.Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling
[J].Development, 2009,136(2):327-335.
[14] Bagri A,Gurney T,He X,et al.The chemokine SDF1 regulates migration of dentate granule cells
[J].Development, 2002,129(18):4249-4260.
[15] Lu M,Grove EA,Miller RJ.Abnormal development of the hippocampal dentate gyrus in mice lacking the CXCR4 chemokine receptor
[J]. Proc Natl Acad Sci USA, 2002, 99(10):7090-7095.
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