Objective　To provide anatomic data for the localization of arytenoid cartilage during clinical operation of arytenoid cartilage.　Methods　After dissecting larynx, the locating relationship between arytenoid and thyroid lamina and the morphological features of the projective point of the muscular process in thyroid lamina and vocal process were observed and measured on 30 normal laryngeal specimens (60 sides). Results　(1)10 parameters of arytenoid were measured, including the distance from the top to bottom of arytenoid. Except for the depth of thyroarytenoid attachment in vocal process, the other parameters were gender significant (P<0.05).  (2)The distance from the muscular process to the lower point of the inferior cornu of thyroid was about (13.05±1.92) mm in male and (11.76±1.79) mm in female and to the posterior edge of thyroid lamina was (8.11±1.17) mm in male and (6.95±1.26) mm in female, and to the medial surface of thyroid lamina was 3.71 mm±1.31 in male and (3.65±1.23) mm in female. (3) The projective point of the muscular processes located in the inferior part of posterior oblique line region of thyroid lamina. The point was far from the posterior edge of larynx with the distance of (7.27±1.52)mm in male and (5.81±1.47) mm in female, from the inferior edge of thyroid lamina with the distance of (7.12±1.43)mm in male and (5.92±1.26) mm in female. Conclusions　 Operation about arytenoids may approach along posterior, inferior edge of thyroid cartilage and inferior edge of cricothyroid joint to locate the muscluar process of arytenoid. As shortest is the distance from muscular process of arytenoid to endosteum of thyroid lamina，if we approach from the posterior oblique line region of thyroid lamina to locate the arytenoid in thyroid cartilage windowing operation, the distance of operation should be shortened..