Objective　To study the communication between the subperitoneal space of the transverse mesocolon (SpST) and the anterior pararenal space.　Methods　Four cadavers (including 3 males, 1 female) were carried out latex injection into the anterior pararenal space, and then undergo CT scanning. After that, sectional specimens (including the transverse and sagittal sections) were performed. The data of CT images, sectional specimens, and the latex diffusion extent in SpST and anterior pararenal space were observed and analyzed.　Results　The latex perfused into the SpST, and encased the middle colonic vessels. In the right portion of SpST, latex got to the body or/and verge of the SpST, while, int the left portion of SpST, latex only diffused to the body, with no reaching to the verge.　Conclusions　SpST communicates with the anterior pararenal space. The relation between SpST and anterior pararenal space can be showed clearly through the latex injection and CT scan.