Objective To establish a more advantageous and suitable middle cerebral artery occlusion (MCAO) model for the study of cervical sympathetic trunk transection in ischemic stroke through the common carotid artery (CCA) approach. Methods Forty rats were randomly allocated into the ECA group and the modified CCA group, and MCAO models were prepared in each group. The operative time, model success rate, Longa grade, infarct rate, and postoperative survival were assessed. Then twelve rats were randomly allocated into the CCA group and the CCA+CSB group. The latter group underwent cervical sympathetic nerve trunk transection. The Longa grade, infarct rate, and protein expression of BCL-2, BAX, and Caspase-3 were evaluated postoperatively in both groups. Results Compared with the ECA group, the modified CCA group showed higher model success rate (P<0.05), higher Longa score (P<0.01), and higher infarct rate (P<0.01), while shorter total operative time (P<0.001). There was no significant difference in postoperative survival between the two groups. Compared with the CCA group, the CCA+CSB group exhibited a lower Longa score (P<0.05) and lower infarct rate (P<0.05), increased BCL-2 expression (P<0.01), and decreased BAX (P<0.05) and Caspase-3 (P<0.05) expression. Conclusions The modified CCA model demonstrates significant advantages over the classic ECA model and is expected to serve as an ideal model for the study of cervical sympathetic trunk transection in ischemic stroke.
Key words
Ischemic stroke /
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Carotid artery approach /
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Middle cerebral artery occlusion modified model /
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Cervical sympathetic trunk transection
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