Objective To explore the molecular mechanisms underlying the development of cholangiocarcinoma. Methods The dataset GSE89749 was downloaded from the GEO database, and differential gene analysis, GO/KEGG enrichment analysis, and protein-protein interaction (PPI) analysis were sequentially performed to identify key genes. The expressions of COL4A1 in 16 types of cancers, including adrenocortical carcinoma, bladder urothelial carcinoma, and invasive breast carcinoma, were analyzed in the TCGA database. Results Differential gene and GO/KEGG enrichment analysis showed that differential genes were significantly enriched in the extracellular matrix containing collagen. PPI analysis identified the gene COL4A1 as a key gene. COL4A1 was found to be highly expressed in cholangiocarcinoma, colorectal cancer, and esophageal cancer among 8 types of cancers. Conclusions COL4A1 is significantly over-expressed in cholangiocarcinoma tissues, providing new perspective and experimental basis for exploring the molecular mechanisms of cholangiocarcinoma development.
Key words
Cholangiocarcinoma /
/
/
GEO database /
/
/
TCGA database /
/
/
COL4A1
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Wu Z, Jiang S, Chen Y. Non-coding RNA and Drug resistance in cholangiocarcinoma[J]. Noncoding RNA Res, 2024, 9(1):194-202. DOI:10.1016/j.ncrna.2023.11.003.
[2] Tomishima K, Suzuki A, Ito K, et al. Remarkable tumor shrinkage in hilar biliary cholangiocarcinoma confirmed by peroral cholangioscopy following neoadjuvant chemotherapy[J]. Endoscopy, 2024, 56(S 01):E85-e86. DOI:10.1055/a-2234-4355.
[3] Rizvi S, Khan SA, Hallemeier CL, Kelley RK, Gores GJ. Cholangiocarcinoma - evolving concepts and therapeutic strategies[J]. Nat Rev Clin Oncol, 2018,15(2):95-111. DOI:10.1038/nrclinonc. 2017.157.
[4] Markasz L, Mobini-Far H, Sindelar R. Collagen type IV alpha 1 chain (COL4A1) expression in the developing human lung[J]. BMC Pulm Med, 2024, 24(1):75. DOI:10.1186/s12890-024-02875-4.
[5] Wang Z, Chen C, Shu J, et al. Single-cell N(6)-methyladenosine-related genes function within the tumor microenvironment to affect the prognosis and treatment sensitivity in patients with gastric cancer[J]. Cancer Cell Int, 2024, 24(1):44. DOI:10.1186/s12935-024-03227-2.
[6] Breedveld G, de Coo IF, Lequin MH, et al. Novel mutations in three families confirm a major role of COL4A1 in hereditary porencephaly[J]. J Med Genet, 2006, 43(6):490-495. DOI:10.1136/jmg.2005.035584.
[7] Coste T, Vincent-Delorme C, Stichelbout M, et al. COL4A1/COL4A2 and inherited platelet disorder gene variants in fetuses showing intracranial hemorrhage[J]. Prenat Diagn, 2022, 42(5):601-610. DOI:10.1002/pd.6113.
[8] Kuo DS, Labelle-Dumais C, Gould DB. COL4A1 and COL4A2 mutations and disease: insights into pathogenic mechanisms and potential therapeutic targets[J]. Hum Mol Genet, 2012, 21(R1):R97-110. DOI:10.1093/hmg/dds346.
[9] Kuuluvainen L, Mönkäre S, Kokkonen H, et al. COL4A1 and COL4A2 Duplication Causes Cerebral Small Vessel Disease With Recurrent Early Onset Ischemic Strokes[J]. Stroke, 2021, 52(10):e624-e625. DOI:10.1161/strokeaha.120.033864.
[10]Cui X, Shan T, Qiao L. Collagen type IV alpha 1 (COL4A1) silence hampers the invasion, migration and epithelial-mesenchymal transition (EMT) of gastric cancer cells through blocking Hedgehog signaling pathway[J]. Bioengineered, 2022, 13(4):8972-8981. DOI:10.1080/21655979.2022.2053799.
[11]Wang T, Jin H, Hu J, et al. COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling[J]. J Exp Clin Cancer Res, 2020, 39(1):148. DOI:10.1186/s13046-020-01650-7.
PDF(3558 KB)
