Objective To investigate the effect and mechanism of Bletilla striata polysaccharides on the choke-area of cross-boundary skin flap in rats. Methods Thirty-two SD rats were randomly divided into two groups: a control group, was given physiological saline; a treatment group, was given physiological saline solution of Bletilla striata polysaccharides. The thoracodorsal artery and posterior intercostal artery were ligated surgically, preserving the iliac lumbar artery. The survival rate of the flap was counted at 7 days after operation. The growth of choke artery was observed by angiography. The microvessel density and arterial intraluminal diameter of choke area were observed by HE staining microscope, and the expression level of vascular endothelial growth factor (VEGF) protein in the flap tissue of choke area was detected by Western blot. Results At day 7 after operation, the survival rate of the flap in the treatment group was higher than that in the control group[(86.1±1.4)% vs (61.3±4.0)%, P<0.0001].The diameter of the iliopsoas artery in the treatment group was thicker than that of the control group(681.60±35.48 vs 446.98±21.61μm, P<0.0001), and the blood vessels extended longer towards the distal end of the flap(6.50±0.33 vs 5.41±0.38 cm，P<0.0001). The microvessel density in choke area was higher in the treatment group [(11.8±1.5) vs (6.7±1.3), P<0.0001], with a larger arterial lumen diameter in choke area(60.14±6.99 vs 28.01±5.07μm, P<0.0001). The expression level of VEGF protein increased (P<0.0001). Conclusions Bletilla polysaccharides can promote the survival of flap, through the regulation of angiogenesis, dilation and VEGF expression in the Chokearea.
Key words
Bletilla striata polysaccharide /
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Choke area /
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Cross-boundary flap /
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Angiogenesis
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References
[1] Xu H, Zhang Z, Xia Y, et al. Preliminary exploration: when angiosome meets prefabricated flaps[J]. J Reconstr Microsurg, 2016, 32(9): 683687. DOI: 10.1055/s00361585468.
[2] Gao ZM, Lin DM, Wang Y, et al. Role of the NO/cGMP pathway in postoperative vasodilation in perforator flaps[J]. J Reconstr Microsurg, 2015, 31(2): 107112. DOI: 10.1055/s00341384663.
[3] 仇玉君, 付裕, 苏航, 等. 蒂部动脉位置对跨区穿支皮瓣成活的影响[J]. 中国临床解剖学杂志, 2022, 40(6): 660-664. DOI: 10.13418/j.issn.1001-165x.2022.6.06.
[4] 马岁录, 何志军, 刘涛, 等. 中药单体调控“细胞自噬”防治皮瓣坏死[J]. 中国组织工程研究, 2024, 28(1): 153-158. DOI: 10.12307/2023.755.
[5] 吕洪乐, 张同华, 李倩. 白及多糖药理作用的研究进展[J]. 中国药房, 2015, 26(28): 4014-4016. DOI: 10.6039/j.issn.1001-0408.2015.28.40.
[6] 付瑞娟. 白芨多糖对小鼠乙酸型胃溃疡治疗作用的探究[D]. 西安: 陕西师范大学, 2016.
[7] Zhao Y, Wang QB, Yan S, et al. Bletilla striata polysaccharide promotes diabetic wound healing through inhibition of the NLRP3 inflammasome[J]. Front Pharmacol,2021, 12: 659215. DOI: 10.3389/FPHAR. 2021.659215.
[8] Qing G, Bailei L, Chunling B, et al. Bletilla striata polysaccharides improve hemostatic, antiinflammatory efficacy, and platelet aggregation in gingivitis rat model[J]. Starch‐Stärke, 2020, 73(3-4): 2000185. DOI: 10.1002/star.202000185.
[9] 魏小于, 郑威楠, 楚亚楠, 等. 白及多糖调节JAK2/STAT3通路对脑缺血再灌注大鼠脑损伤和炎症反应的影响[J]. 中国临床药理学杂志, 2020, 36(13):1825-1829.DOI: 10.13699/j.cnki.10016821.2020. 13.014.
[10]颜文斌, 曾祥涛, 戴孟诗, 等. 白芨多糖对围绝经期大鼠卵巢VEGF、血清MDA、子宫内膜形态学的影响[J]. 局解手术学杂志, 2018, 27(10): 698-701. DOI: 10.11659 /jjssx.05E018120.
[11]Wang YP, Zhang X, Zhou M, et al. Irisin pre-treatment promotes multi-territory perforator flap survival in rats: an experimental study[J]. Injury, 2020, 51(11): 2442-2448. DOI: 10.1016/j.injury.2020.07.058.
[12]宋荷花, 张其, 吴必华, 等. Ⅳ级穿支血管与网状血管供血皮瓣成活关系的研究[J]. 中国临床解剖学杂志, 2023, 41(3): 288-295. DOI: 10.13418/j.issn.1001-165x.2023.3.08.
[13]程晟, 习珊珊, 何耀之, 等. 穿支皮瓣Choke区动、静脉血流阻力对皮瓣存活的影响[J]. 中国临床解剖学杂志, 2018, 36(6): 648-656. DOI: 10.13418/j.issn.1001-165x.2018.06.010.
[14]Xi SS, Zhao JF, Kang RC, et al. The effects of angiosome morphology on choke vessels and flap necrosis in a rat multiterritory perforator flap[J]. Ann Plast Surg,2023, 91(4): 479-484. DOI: 10.1097/SAP.000000 0000003643.
[15]史旭锋, 魏冬, 余小平, 等. 血管内皮生长因子介导的血管新生促进创面愈合的研究进展[J]. 中华损伤与修复杂志, 2021, 16(6): 536-538. DOI: 10.3877/cma.j.issn.1673-9450.2021.06.015.
[16]殷杰, 赵胜利, 蔡晓明, 等. 基于Wnt/β-catenin信号通路探讨VEGF基因转染骨髓源内皮祖细胞移植治疗大鼠缺血皮瓣的实验[J]. 中国现代医生, 2021, 59(31): 36-40, 193. DOI: 10.3969/j.issn.1673-9701.2021.31.zwkjzlml-yyws202131009.
[17]Xiao P, Zhu X, Sun JP, et al. Gartilage tissue miR2143p regulates the TrkB/ShcB pathway paracrine VEGF to promote endothelial cell migration and angiogenesis[J]. Bone, 2021, 151: 116034. DOI: 10.1016/j.bone.2021.116034.
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