Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway

doi:10.13418/j.issn.1001-165x.2024.1.09

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Chinese Journal of Clinical Anatomy ›› 2024, Vol. 42 ›› Issue (1) : 47-53. DOI: 10.13418/j.issn.1001-165x.2024.1.09

Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway

Qi Zhi^#, Yang Li^#, Jia Qiuye, Chen Haolun, Duan Zhaoda, Wu Chunyun*

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Abstract

Objective To investigate the effect of edaravone on Sirt3 and MAPKs pathway-related proteins in LPS-activated microglia and its regulatory mechanism. Methods Western Blot and immunofluorescence were used to detect the effect of edaravone on the protein expression of Sirt3 and MAPKs pathway-related proteins ERK1/2, P38, JNK as well as their phosphorylated forms p-ERK1/2, p-P38 and p-JNK in the activated BV2 cells. Western Blot techniques were applied to detect the effects of ERK1/2 pathway inhibitor on the expressions of p-ERK1/2 and Sirt3. Results The expressions of Sirt3, p-ERK1/2, p-P38 and p-JNK in microglia were significantly increased after BV2 cells activation. Edaravone promoted the expression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38. The protein expression of Sirt3 and p-ERK1/2 decreased after co-treatment with edaravone and ERK1/2 inhibitor. Edaravone promoted the overexpression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38. Conclusions Edaravone can regulate MAPKs signaling pathway and promote Sirt3 production in BV2 cells, possibly regulates neuroinflammatory responses via ERK/Sirt3 signaling pathway.

Key words

Edaravone; / / Sirt3; / / Microglia; / / MAPKs signaling pathway

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Qi Zhi, Yang Li, Jia Qiuye, Chen Haolun, Duan Zhaoda, Wu Chunyun. Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway[J]. Chinese Journal of Clinical Anatomy. 2024, 42(1): 47-53 https://doi.org/10.13418/j.issn.1001-165x.2024.1.09

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