Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway

doi:10.13418/j.issn.1001-165x.2024.1.07

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Chinese Journal of Clinical Anatomy ›› 2024, Vol. 42 ›› Issue (1) : 33-41. DOI: 10.13418/j.issn.1001-165x.2024.1.07

Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway

Wang Xinghang1, Ding Jiayuan2, Li Fang3, Bao Cuifen4*, Yan Lijing4*

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Abstract

Objective To investigate the effect of ginsenoside Rg1 on the inflammatory response of microglia cells after oxygen-glucose deprivation/resupply injury through NLRP3 inflammasome pathway, and to further investigate its anti-inflammatory mechanism. Methods BV-2 microglia were randomly divided into six groups: No treatment group (Con), oxygen glucose deprivation/resupply group (OGD/R), ginsenoside Rg1 low, medium and high dose groups (0.1, 0.2, 0.4 mmol/L, Rg1L, Rg1M, Rg1H), MCC950 control group (0.05 mmol/L, MCC950). The cell proliferation was detected by CCK8. Immunofluorescence and western blotting were used to detect the expression of NLRP3 inflammasome-related protein in BV-2 microglia after treatment with different concentrations of ginsenoside Rg1 and MCC950 for 48 h. The expression levels of IL-1β and IL-18 in BV-2 microglia were detected by ELISA. Results Compared with Con group, Iba-1 green fluorescence was observed in the cytoplasm of BV-2 microglia cells treated with hypoxia and glucose deficiency/resupply. After OGD/R treatment of BV-2 microglia for 2h, the proliferation rate of BV-2 microglia cells showed an obvious downward trend after 48 h culture with different concentrations of ginsenoside Rg1 and MCC950. There were significant positive expressions of NLRP3, ASC, Caspase-1, IL-1β and IL-18 in the OGD/R group. The NLRP3 protein expression in Rg1L, Rg1M and Rg1H groups was significantly decreased, and the expression decreased with the increasing of drug concentration. The results showed that compared with OGD/R group, ginsenoside Rg1 and MCC950 inhibited the expression of NLRP3 in activated BV-2 microglia cells (P<0.01). Conclusions Ginsenoside Rg1 may inhibit the expression levels of IL-1β and IL-18, thus inhibiting the inflammatory response by inhibiting the expressions of NLRP3, ASC, Caspase-1 and interfering with NLRP3 inflammasome synthesis.

Key words

Ginsenoside Rg1; / / Oxygen and glucose deprivation/resupply; / / Microglia; / / Inflammation; / / NLRP3

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Wang Xinghang, Ding Jiayuan, Li Fang, Bao Cuifen, Yan Lijing. Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway[J]. Chinese Journal of Clinical Anatomy. 2024, 42(1): 33-41 https://doi.org/10.13418/j.issn.1001-165x.2024.1.07

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