Objective To explore whether Fasudil can promote the regeneration of the sensory nerve after flap surgery and its underlying mechanism. Methods 92 ICR mice were selected and randomly divided into the control, Fasudil , LY294002, and Fasudil+LY294002 groups, and the corresponding drugs were injected intraperitoneally daily from 3d before surgery. At 0 d, 5 d, 7 d, 15 d and 30 d after operation, immunofluorescence staining was used to observe the axon outgrowth in the flap. The flap tissue at 5d after surgery was collected, and Western blotting was used to detect the expressions of RhoA, ROCK1+2, p-CPI-17, p-MYPT, p-PTEN, p-PI3K, and p-Akt in the flap. Secondly, rats 15d in pregnancy were adopted, the DRGs on both sides of the spinal cord were harvested and divided into 6 groups: the control, Fasudil, Fasudil+LY294002, CSPG, CSPG+Fasudil and CSPG+Fasudil+LY294002 groups. After 5d of culture, the axon lengths of the DRGss in the 6 groups were measured. Results Abundant nerve fibers could be observed in each group at 0d after operation; at 7d after operation, all nerve fibers underwent complete degeneration; at 15d and 30d after operation, only the Fasudil group had nerve fibers again. p-CPI-17 and p-MYPT in Fasudil group were down-regulated, and p-PI3K and p-Akt were up-regulated 5 days after operation (P<0.001). The average axon lengths of DRGs in the control, Fasudil, Fasudil+LY294002, CSPG, CSPG+Fasudil, and CSPG+Fasudil+LY294002 groups after 5d of culture were (1.96±0.24) mm, (2.73±0.30)mm, (2.07±0.13)mm, (1.62±0.20)mm, (2.35±0.13)mm, and (1.84±0.31)mm, respectively. The differences among the groups were statistically significant (P<0.001). Conclusions Fasudil promotes nerve regeneration in flap after surgery by inhibiting the RhoA/ROCK pathway and activating the PI3K/Akt pathway.
Key words
Flap /
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Nerve regeneration /
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Fasudil /
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RhoA/ROCK /
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PI3K/Akt
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