Objective To investigate the protective and regulative mechanism of Ginsenoside Rg1 on acute kidney injury (AKI) induced acute lung injury. Methods Sixty male Kunming mice were randomly divided into 4 groups with 15 mice per group: Group A (sham operation group), Group B (model group), Group C (Ginsenoside Rg1 group), and Group D (necrostatin-1 control group). AKI models were prepared，after 24h, the serum level of serum creatinine (Scr) and blood urea nitrogen (BUN) were detected. The wet/dry weight ratio of lung tissues were also measured. Pathological changes of lung tissues were evaluated by HE staining. Expression of tumor necrosis factor-α (TNF-α), IL-1β, IL-6 and IL-8 were detected by ELISA. Expressions of tumor necrosis factor receptor 1 (TNFR1) and receptor interacting protein kinase (RIPK) were observed by immunohistochemistry and western blotting. Results (1) Compared with Group A, the serum level of Scr and BUN in Group B were significantly increased, while compared with Group B, the serum level of Scr and BUN in Group C and Group D were significantly decreased (P<0.01). (2) Compared with Group A, the wet/dry ratio of lung tissues in Group B was significantly increased. Compared with Group B, the wet/dry ratio of lung tissues in Group C and Group D was significantly decreased (P<0.01). HE staining showed that the lung tissues structure of Group A was basically intact, while part of alveolar cavity was collapsed, cells were necrotic with the serious infiltration of inflammatory cells in Group B. Compared with Group B, the lung injury degree of Group C and Group D was alleviated. (3) ELISA showed that TNF-α, IL-1β, IL-6 and IL-8 in serum of Group B were significantly increased compared with Group A, while in Group C and Group D were significantly decreased compared with Group B (P<0.01). (4) Immunohistochemical and western blotting results showed that the protein content of TNFR1, RIPK1 and RIPK3 in Group B were significantly increased compared with Group A, while in Group C and Group D were significantly decreased compared with Group B (P<0.01). Conclusions Ginsenoside Rg1 can alleviate the acute lung injury induced by acute kidney injury, the mechanism may be related to the inhibition of TNF-α/TNFR1/RIPKs pathway.
Key words
Ginsenoside Rg1; /
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TNF-α/TNFR1/RIPKs; /
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AKI; /
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ALI /
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