Objective To investigate the mechanism of gastrodin regulating the activation of BV2 cells through Notch signal pathway. Methods BV2 cells were cultured in vitro. Western Blot and immunofluorescence double staining were used to detect the effect of gastrodin on the protein expression of Notch-1 and intracellular Notch receptor domain (NICD) , recombining binding protein suppressor of hairless (RBP-JK) and transcription factor hairy and enhancer of split-1 (Hes-1) in activated BV2 cells induced by oxygen-glucose deprivation (OGD). Results Western Blot results showed that the expression of Notch-1, NICD, Hes-1 and RBP-JK proteins was increased in OGD induced BV2 cells, and gastrodin treatment decreased the expression of these protein expressions. Double immunofluorescence staining showed that gastrodin could significantly reduce the expression of Notch-1, NICD, Hes-1 and RBP-JK proteins in activated microglia. Conclusions Gastrodin can regulate the activation of BV2 cells through Notch signal pathway to play its protective role.
Key words
Microglia; /
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Oxygen glucose deprivation; /
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Gastrodin; /
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Notch signaling pathway
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