Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice&nbsp;

doi:10.13418/j.issn.1001-165x.2023.3.10

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Chinese Journal of Clinical Anatomy ›› 2023, Vol. 41 ›› Issue (3) : 304-309. DOI: 10.13418/j.issn.1001-165x.2023.3.10

Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice

Zhou Qian¹, Li Lixia¹, Yang Xiaoxia¹, Yang Junhua¹, Wen Jinkun¹, Li Pengfei², Luo Li¹, Li Guoying¹*

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Abstract

Objective To investigate the effect of Macamide B on the expression of progranulin (PGRN) in neurons of neonatal mice with hypoxic-ischemic brain damage (HIBD) and HT22 mouse hippocampal neuron cell line after oxygen-glucose deprivation (OGD) and explore the mechanism of the neuroprotective effect of Macamide B after HIBD in neonatal mice. Methods The HIBD model of C57 mice was established after 7 days of birth and the mice were divided into Sham group, HIBD group and Macamide B group. HT22 cells were cultured, then the OGD model of HT22 cells was established, and the cells were divided into Control group, OGD group and Macamide B group. The expression of PGRN in mouse neurons or HT22 cells was detected by immunofluorescence staining and Western blot. Results In vivo experiments showed that compared with Sham group, the expression of PGRN in mice neurons of HIBD group was reduced. Compared with HIBD group, the expression of PGRN in neurons of Macamide B group was increased. In vitro, compared with Control group, the expression of PGRN in HT22 cells of OGD group was decreased. Compared with OGD group, the expression of PGRN in HT22 cells of Macamide B group rose significantly. Conclusions Macamide B may play a neuroprotective role in HIBD neonatal mice by promoting the expression of PGRN.

Key words

Hypoxic-ischemic brain damage; / / Macamide B; / / Neuroprotection; / / PGRN

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Zhou Qian, Li Lixia, Yang Xiaoxia, Yang Junhua, Wen Jinkun, Li Pengfei, Luo Li, Li Guoying. Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice [J]. Chinese Journal of Clinical Anatomy. 2023, 41(3): 304-309 https://doi.org/10.13418/j.issn.1001-165x.2023.3.10

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