Objective To explore the differentially expressed genes of the suture dura compared with the normal subcranial dura and the mechanism of the suture dura mater on the migration of cranial suture cells, so as to provide theoretical basis for clinical suture premature closure. Methods Six 51-day- old Ovis aries were selected. The dura mater under the cranial suture and non-cranial suture were extracted, respectively. The second-generation sequencing was used to detect the differential expression of mRNA in the dural cells of different parts of the Ovis aries in the developmental stage. The biological information were analyzed. The changes of mRNA expression in the sequencing results were verified by Real-time PCR. Results Through the analysis of the biological information of the differential expression up-regulation genes shared by the dura mater at the suture and the dura below the skull, it was found that the up-regulation of the dura at the suture, relative to the dura below the skull, was mainly focused on cell migration, vascular bundle formation, and extracellular matrix production. The most enriched cell pathway was PI3K-AKT cell signaling pathway. Validation of the PI3K signaling pathway gene with high expression in the dura showed that the expression of PI3K-AKT cell signaling pathway in the dura below the suture was higher than that of the normal skull, and it was statistically significant (P<0.05). Conclusions The PI3K-AKT cell signaling pathway expression level in the dura at the suture is significantly increased compared with that below the skull, which may be related to the proliferation and migration function of the PI3K-AKT cell signaling pathway.
Key words
Skull development; /
/
Cranial suture; /
Dura materm; /
mRNA; /
Second-generation sequencing technology
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