Objective To investigate the anti-inflammatory effect of sargentodoxa cuneata decoction (SCD) after spinal cord injury (SCI) and its effect on the recovery of motor function. Methods Thirty-six SPF grade mice were randomly divided into a sham operation group, a SCI group and a SCD group. The recovery of hindlimb motor function was detected by BMS (Basso mouse scale), and the expression levels of interleukin (IL) -6, IL-12A, tumor necrosis factor (TNF) -α and brain-derived neurotrophic factor (BDNF) were detected by Western blot. Immunofluorescence staining of NeuN was performed to observe the changes of neurons in anterior horn of spinal cord. The distribution of spinal cord motoneurons related to tibialis anterior muscle (TA) was observed by rAAV-retro retrograde tracing. Results Compared with SCI group, the BMS score of SCD group was significantly higher at 7 days after injury (P<0.05), and IL-6, IL-12A and TNF-α were significantly higher at 14 days after injury, while the expression of BDNF was significantly higher than that in SCI group (P<0.05). Compared with SCI group, the number of spinal cord anterior horn neurons in SCD group was significantly increased (P<0.05), and the number of rAAV-retro retrograde labeled TA related motor neurons was also significantly increased (P<0.05). Conclusions The sargentodoxa cuneata decoction can significantly down-regulate the levels of inflammatory factors IL-6, IL-12A and TNF- α to play an anti-inflammatory role and up-regulate the expression of BDNF, so as to protect motor neurons and promote the recovery of motor function in mice after spinal cord injury.
Key words
Spinal cord injury; /
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Sargentodoxa cuneata; /
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Anti-inflammatory effect; /
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Functional recovery
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