Action mechanism of pinoresinol diglucoside on improving osteoporosis in mice based on Nrf2 pathway

doi:10.13418/j.issn.1001-165x.2023.2.08

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Chinese Journal of Clinical Anatomy ›› 2023, Vol. 41 ›› Issue (2) : 162-171. DOI: 10.13418/j.issn.1001-165x.2023.2.08

Action mechanism of pinoresinol diglucoside on improving osteoporosis in mice based on Nrf2 pathway

Huang Xingxiang¹, Zhong Chao²*, Ye Hua², Xu Chunyi³, Liu Yunfei⁴, Jiang Lu⁴

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Abstract

Objective To analyze the action mechanism of pinoresinol diglucoside (PD) on improving osteoporosis. Methods The trabecular bone volume/total volume (BV/TV), average trabecular thickness (Tb.Th), average number of trabeculae (Tb.N) and average trabecular spacing (Tb.Sp) were analyzed by micro-CT. The damage of bone tissues was detected by HE staining. Number of bone cells/bone circumference (N.Ob/B.Pm), vacuole rate and osteoclast surface/bone surface (Ocs/BS) were analyzed by TRAP staining. The relative activities of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected by ELISA. The expressions of Nuclear factor erythroid-2-related factor 2 (Nrf2) and HO-1 were detected by Western Blotting. Results Compared with sham operation group, bone loss was increased in model group, BV/TV, Tb.Th, Tb.N, N.Ob/B.Pm, relative activity of ALP, SOD, GSH, Nrf2 and HO-1 expression levels were significantly decreased, Tb.Sp, vacuole rate, Ocs/BS, relative activity of TRAP and MDA level were significantly increased (P<0.05). The changes in E2 group and PD group were completely opposite to those in model group (P<0.05). There was no significant difference in the above indexes between PD group and E2 group (P>0.05). Conclusions PD can improve osteoporosis in mice by activating Nrf2 signaling pathway.

Key words

Pinoresinol diglucoside; / Osteoporosis; / Nuclear factor erythroid-2-related factor 2 / Osteoblast; / / Osteoclast; / / Oxidative stress

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Huang Xingxiang, Zhong Chao, Ye Hua, Xu Chunyi, Liu Yunfei, Jiang Lu. Action mechanism of pinoresinol diglucoside on improving osteoporosis in mice based on Nrf2 pathway[J]. Chinese Journal of Clinical Anatomy. 2023, 41(2): 162-171 https://doi.org/10.13418/j.issn.1001-165x.2023.2.08

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