Objective To study the effects of Kushen gel on histopathology, interleukin -6 (IL-6) and protease activated receptor -2 (PAR-2) levels in psoriatic rats. Methods Sixty Wistar rats were divided into a control group, a model group, a vitamin A acid group, Kushen gelatin high, medium and low dose groups, 10 rats in each group. The general condition, pathological changes of skin lesions, inflammatory cell infiltration score, Baker score and levels of IL-6 and PAR-2 in serum and skin lesions were observed in each group. Results The scales and erythema on the back of rats in each group treated with vitamin A acid and Sophora flavescens gel were alleviated than those in model group. The symptom relief degree of Kushen gel group was better than that of vitamin A acid group. The pathological changes of the skin lesions of rats in different doses of vitamin A acid group and Sophora flavescens gel group were improved to a certain extent than those in model group. The improvement of Kushen gel medium and high dose group was better than that of vitamin A acid group. The inflammatory cell infiltration score, Baker score, IL-6 and PAR-2 levels in the model group were significantly higher than those in the control group (P<0.05). The inflammatory cell infiltration score, Baker score, IL-6 and PAR-2 levels in each treatment group were significantly lower than those in the model group (P<0.05). The inflammatory cell infiltration score, Baker score, IL-6 and PAR-2 levels in the middle and high dose group were significantly lower than those in the A acid group (P<0.05). Conclusions Kushen gel can improve histopathology and IL-6 and PAR-2 levels in psoriatic rats.
Key words
Sophora flavescens gel; /
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Psoriasis; /
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rat; /
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Interleukin -6; /
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Protease activated receptor -2
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References
[1] 曹雪琛, 宋乐彬, 鲁严, 等. 银屑病发病机制中IL-17关键轴与IgE的相互作用[J]. 国际免疫学杂志, 2021, 44(1): 105-109. DOI: 10.3760/cma.j.issn.1673-4394.2021.01.019.
[2] 王若珺, 李若瑜. 白细胞介素17A及其受体拮抗剂治疗银屑病的不良反应[J]. 中华皮肤科杂志, 2021, 54(2): 170-173. DOI: 10.35541/cjd.20190858.
[3] 王圳伊, 王露露, 张晶. 苦参的化学成分, 药理作用及炮制方法的研究进展[J]. 中国兽药杂志, 2019, 53(10): 75-83. DOI: 10.11751/ISSN.1002-1280.2019.10.10.
[4] 杜惠兰, 魏绍斌, 谈勇, 等. 苦参凝胶临床应用指导意见[J]. 中草药, 2020, 11(8): 23-24. DOI: 10.7501/j.issn.0253-2670.2020.08.014.
[5] 吕宏蓬, 刘欣, 孟旭, 等. 苦参在银屑病中的应用及研究进展[J]. 天津中医药大学学报, 2019, 2(3): 300-304. DOI: 10.11656/j.issn.1673-9043.2019.03.22.
[6] 魏荣, 吴斌, 吕静. 白细胞介素-6抑制剂对银屑病模型大鼠背部皮肤组织的影响及作用机制[J]. 中国老年学杂志, 2020, 40(4): 856-860. DOI: 10.3969/j.issn.1005-9202.2020.04.057.
[7] 黄丹丹, 刘智敏, 赵严冬, 等. 芍倍注射液对痔急性发作模型大鼠直肠肛管组织病理形态学, 炎症反应的影响研究[J]. 结直肠肛门外科, 2020, 26(4): 67-71. DOI: 10.19668/j.cnki.issn1674-0491.2020.04.014.
[8] 张丽娟. 中西医结合治疗治疗寻常型银屑病50例疗效观察[J]. 中国中西医结合皮肤性病学杂志, 2019, 18(5): 81-82. DOI: 10.3969/j.issn.1672-0709.2019.05.023.
[9] 宫春明, 王利梅, 祁凤华, 等. 从中医经典毒邪论洽银屑病探析[J]. 河北中医药学报, 2020, 1(2): 12-15. CNKI: SUN:HZYX.0.2020-02-004.
[10] 丁久云. 活血解毒汤联合复方甘草酸苷胶囊治疗寻常型银屑病56例疗效分析[J]. 四川中医, 2020, 3(2): 169-171. CNKI: SUN:SCZY. 0.2020-02-060.
[11] 张明发, 沈雅琴. 氧化苦参碱对皮肤疾病的药理作用与临床应用研究进展[J]. 药物评价研究, 2020, 5(4): 101-105. DOI: 10.7501/j.issn.1674-6376.2020.04.038.
[12] 黄凤娇, 吴森, 王平慧, 等. 复方苦参洗剂的安全性研究[J]. 医药导报, 2020, 343(5): 24-26. DOI: 10.3870/j.issn.1004-0781.2020.05.005.
[13] 殷彩苗, 魏绍斌, 黄利, 等. 苦参凝胶治疗宫颈高危型人乳头瘤病毒感染的Meta分析[J]. 中草药, 2020, 671(12): 157-162. DOI: 10.7501/j.issn.0253-2670.2020.12.019.
[14] Hongpeng LV, Xin L, Xu M, et al. Progress of sophora flavescens in psoriasis treatment[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2019, 6(13): 126-130. DOI: 10.20996/1819-6446-2019-8-1-95-102.
[15] Wang M, Kang X, Deng LL, et al. Deep eutectic solvent assisted synthesis of carbon dots using sophora flavescens aiton modified with polyethyleneimine: application in myricetin sensing and cell imaging[J]. Food Chem, 2021, 345(8): 128817-128820. DOI: 10.1016/j.foodchem.2020.128817.
[16] 朱思庆, 庞璐璐, 马金秋, 等. 苦参凝胶对小鼠慢性湿疹的治疗作用[J]. 国际药学研究杂志, 2020, 47(9): 52-58. DOI: 10.13220/j.cnki.jipr.2020.09.008.
[17] 刘盼盼, 张子锐, 杨旭, 等. 姜黄素减轻小鼠深部组织压力性损伤[J]. 基础医学与临床, 2020, 40(3): 299-304. DOI: 10.3969/j.issn.1001-6325.2020.03.003.
[18] 徐文静, 席建元. 银屑平丸对BALB/c小鼠银屑病样模型血清中IL-6与IL-21表达的影响[J]. 中医药导报, 2018, 24(22): 17-20. CNKI: SUN:HNZB.0.2018-22-006.
[19] Basile G, Vetere A, Liu KC, et al. 111-OR: pancreatic elastase regulates human beta-cell proliferation via the focal adhesion and PAR2 signaling pathways[J]. Diabetes, 2019, 68(1): 111-113. DOI: 10.2337/db19-111-OR.
[20]王桂萍, 武宇, 李虎虎. 白斑膏对瘙痒模型小鼠PAR-2和组胺水平的影响[J]. 山西中医, 2019, 35(6): 56-58. DOI: CNKI: SUN:SHIX.0.2019-06-026.
[21] Carey RM, Freund JR, Hariri BM, et al. Polarization of protease-activated receptor 2 (PAR-2) signaling is altered during airway epithelial remodeling and deciliation[J]. J Biol Chem, 2020, 295(19): 2710-2715. DOI: 10.1074/jbc.RA120.012710.
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