Effect of Scutellarin on M2-type polarization in activated BV-2 microglia via STAT3 signaling

doi:10.13418/j.issn.1001-165x.2023.1.08

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Chinese Journal of Clinical Anatomy ›› 2023, Vol. 41 ›› Issue (1) : 36-44. DOI: 10.13418/j.issn.1001-165x.2023.1.08

Effect of Scutellarin on M2-type polarization in activated BV-2 microglia via STAT3 signaling

Jia Qiuye, Zhang Xiaolina, Chen Haolun, Yuan Yun, Wu Chunyun

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Abstract

Objective To investigate the role of scutellarin on the polarization of M2 microglia under the action of lipopolysaccharide (LPS) and the regulation mechanism of signal transducers and activators of transcription 3 (STAT3). Methods BV-2 microglia were divided into a control group (Con), a STAT3 inhibitor α-cyano-(3,4-dihydroxy)-N-benzylmycin-nicotinamide group (AG490), a LPS group (LPS), a LPS+AG490 group, a LPS+scutellarin pretreatment group (LPS+S), a LPS+S+AG490 group. The expression of STAT3, phosphorylated STAT3 (P-STAT3) and the M2 microglia marker interleukin 10 (IL-10) were detected by Western Blot and immunofluorescence staining. Results Western Blot results showed: the expression of P-STAT3 and IL-10 in the LPS group was significantly enhanced, compared with that in the control group (P<0.05); the expression of P-STAT3 was significantly reduced, the expression of IL-10 was obviously increased after pretreatment with scutellarin; both were significantly different from the LPS group (P<0.05). AG490 enhanced the effect of scutellarin. In addition, there was no statistical difference s in STAT3 in each group (P>0.05). Immunofluorescence staining results showed that scutellarin intervention reduced protein level of P-STAT3 in LPS-activated BV-2 microglia, enhanced protein level of IL-10. There was no statistical difference in protein level of STAT3 in each group. Conclusions Scutellarin promotes the polarization of BV-2 microglia to M2 type by inhibiting the activation of STAT3, which may be related to the effect of scutellarin in reducing neuro-inflammatory responses.

Key words

Scutellarin; / / BV-2 microglia; / / STAT3; / / Polarization

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Jia Qiuye, Zhang Xiaolina, Chen Haolun, Yuan Yun, Wu Chunyun. Effect of Scutellarin on M2-type polarization in activated BV-2 microglia via STAT3 signaling[J]. Chinese Journal of Clinical Anatomy. 2023, 41(1): 36-44 https://doi.org/10.13418/j.issn.1001-165x.2023.1.08

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