Objective To investigate the antioxidant and protective effect of tanshinone IIA (Tan ⅡA) on CCl4-induced acute liver injury in mice and its possible mechanism. Methods C57BL/6J mice were randomly divided into a control group, a CCl4 group and a Tan ⅡA protective group (Tan ⅡA 20 mg/kg+CCl4), with 10 mice in each group. Acute liver injury model was induced by intraperitoneal injection of CCl4 in mice. The liver indices were calculated, the activities of serum AST and ALT were detected, the activity of SOD and the contents of GSH and MDA in liver tissue were measured, and the pathological changes of liver tissue were observed by HE staining. The expression levels of PI3K, p-PI3K, Akt, p-Akt, Nrf2 and HO-1 proteins in liver tissue were detected by immunohistochemistry and Western blot. Results Compared with the CCl4 group, in the Tan ⅡA protective group, the liver indices was significantly decreased (P<0.01), the activities of serum AST (P<0.01) and ALT (P<0.05) was reduced, the activity of SOD (P<0.01) and the content of GSH (P<0.05) were increased while decreasing the content of MDA (P<0.05) in liver tissue, and the pathological changes of liver tissue was significantly improved. In addition, Tan ⅡA could significantly increase the expression levels of p-PI3K and p-Akt (P<0.01) in liver tissue, while significantly inducing Nrf2 translocation into nucleus (P<0.01), which significantly increased the expression level of its downstream target protein HO-1 (P<0.01). Conclusions Tan ⅡA can significantly ameliorate CCl4-induced acute liver injury. Its mechanism may be related to PI3K/Akt/Nrf2/HO-1 signaling pathway.
Key words
Tanshinone ⅡA /
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Acute liver injury /
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PI3K/Akt/Nrf2/HO-1 pathway /
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Antioxidation
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